Simple Plex Discovery Human M-CSF R

Name: Simple Plex Discovery Human M-CSF R
Brand: ProteinSimple
SKU: SPCKB-PS-015350

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-015350

Simple Plex Human M-CSF R assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Cerebrospinal Fluid (5 uL)
Sensitivity:	4.7 pg/mL
Assay Range:	8.39 - 80,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Discovery Human M-CSF R Summary:	Simple Plex Discovery assay is for the detection of human Macrophage Colony Stimulating Factor Receptor (M-CSF R) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Discovery Human M-CSF R

Technical Data
Citations

Product Specifications for Simple Plex Discovery Human M-CSF R

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human M-CSF R.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one M-CSF R assay

Inter-Assay Precision (Precision between assays) Replicates of each M-CSF R control were tested in multiple assays performed by at least three technicians using two lots of reagents for human M-CSF R

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	30	30
Mean (pg/mL)	663	33859	614	31629
Standard Deviation	23.1	2316	55.2	2672
CV%	3.5	6.8	9	8.4

Recovery

Recovery for the human M-CSF R assays was evaluated at three different spiked concentrations of M-CSF R protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	96	93-100

Linearity

Samples containing and/or spiked with high concentrations of M-CSF R were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Discovery Human M-CSF R

	Simple Plex Discovery Human M-CSF R Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human M-CSF R Assay. Assay range is 8.39-80,000 pg/mL.

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: M-CSF R/CD115

M-CSF R/CD115, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes c-kit, the receptor for SCF, and the alpha and beta receptors for PDGF. M-CSF R/CD115 is expressed primarily on cells of the monocyte/macrophage lineage and in various tissues of the developing placenta.

Item	Value
Long Name	Macrophage Colony Stimulating Factor Receptor
Entrez Gene IDs	1436 (Human); 12978 (Mouse)
Alternate Names	CD115, CD115 antigen, CSF-1-R, CSF1R, CSFR, EC 2.7.10.1, FMS proto-oncogene, FMSFIM2, M-CSF R, M-CSFR, MCSFR, McDonough feline sarcoma viral (v-fms) oncogene homolog, Proto-oncogene c-Fms, c-fms, colony stimulating factor 1 receptor, macrophage colony stimulating factor I receptor, macrophage colony-stimulating factor 1 receptor

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Discovery Human M-CSF R

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Protocols & Manuals

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