Simple Plex Discovery Human Complement Factor D Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012122
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human Complement Factor D Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Complement Factor D assay.
Inter-Assay Precision (Precision between assays) Replicates of each Complement Factor D control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Complement Factor D.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 33 | 33 |
| Mean (pg/mL) | 355 | 18708 | 352 | 17948 |
| Standard Deviation | 25.0 | 847 | 40.8 | 1600 |
| CV% | 7.0 | 4.5 | 11.6 | 8.9 |
Recovery for Simple Plex Discovery Human Complement Factor D Assay
Recovery for the human Complement Factor D assays was evaluated at three different spiked concentrations of Complement Factor D protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 111 | 111-112 |
Linearity
Samples containing and/or spiked with high concentrations of Complement Factor D were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human Complement Factor D Assay
Simple Plex Discovery Human Complement Factor D Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human Complement Factor D Assay. Assay range is 12.0-45,680 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Complement Factor D/Adipsin
Complement Factor D, also known as adipsin, is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3.3 Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors.
Alternate Names
Gene Symbol
Additional Complement Factor D/Adipsin Products
Product Documents for Simple Plex Discovery Human Complement Factor D Assay
Product Specific Notices for Simple Plex Discovery Human Complement Factor D Assay
For research use only