Simple Plex Discovery Human CD28 Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012119
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human CD28 Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 29 | 29 |
| Mean (pg/mL) | 1563 | 54643 | 1233 | 56044 |
| Standard Deviation | 115 | 4599 | 142 | 5087 |
| CV% | 7.4 | 8.4 | 11.5 | 9.1 |
Recovery for Simple Plex Discovery Human CD28 Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 102 | 99-105 |
| EDTA Plasma (n=2) | 82 | 78-86 |
| Heparin Plasma (n=2) | 95 | 94-97 |
| Serum (n=2) | 85 | 83-86 |
Linearity
Samples containing and/or spiked with high concentrations of human CD28 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human CD28 Assay
Simple Plex Discovery Human CD28 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: CD28
CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes.
Alternate Names
Gene Symbol
Additional CD28 Products
Product Documents for Simple Plex Discovery Human CD28 Assay
Product Specific Notices for Simple Plex Discovery Human CD28 Assay
For research use only