Simple Plex Discovery Human CD23/Fc epsilon RII Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012700
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human CD23/Fc epsilon RII Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 23 | 23 |
| Mean (pg/mL) | 96.1 | 4470 | 107 | 4782 |
| Standard Deviation | 4.23 | 206 | 11.1 | 312 |
| CV% | 4.4 | 4.6 | 10.3 | 6.5 |
Recovery for Simple Plex Discovery Human CD23/Fc epsilon RII Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 104 | 103-106 |
Linearity
Samples containing and/or spiked with high concentrations of human CD23 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human CD23/Fc epsilon RII Assay
Simple Plex Discovery Human CD23/Fc epsilon RII Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: CD23/Fc epsilon RII
CD23, also known as Fc epsilon RII, is a type 2 transmembrane C-type lectin that binds IgE, CD21, CD11b and CD11c. It is expressed on a variety of hematopoietic cells and functions in allergic immune responses.
Long Name
Alternate Names
Gene Symbol
Additional CD23/Fc epsilon RII Products
Product Documents for Simple Plex Discovery Human CD23/Fc epsilon RII Assay
Product Specific Notices for Simple Plex Discovery Human CD23/Fc epsilon RII Assay
For research use only