Simple Plex Discovery beta-Defensin 2 Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-014655
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery beta-Defensin 2 Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one beta-Defensin 2 assay.
Inter-Assay Precision (Precision between assays) Replicates of each beta-Defensin 2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human beta-Defensin 2.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 50 | 52 |
| Mean (pg/mL) | 471 | 22753 | 384 | 20887 |
| Standard Deviation | 18.3 | 655 | 44.0 | 1988 |
| CV% | 3.9 | 2.9 | 11.4 | 9.5 |
Recovery for Simple Plex Discovery beta-Defensin 2 Assay
Recovery for the human beta-Defensin 2 assays was evaluated at three different spiked concentrations of beta-Defensin 2 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 91 | 87-94 |
| EDTA Plasma (n=2) | 90 | 78-102 |
| Heparin Plasma (n=2) | 100 | 82-117 |
| Serum (n=2) | 105 | 91-120 |
Linearity
Samples containing and/or spiked with high concentrations of beta-Defensin 2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery beta-Defensin 2 Assay
Simple Plex Discovery Human beta-Defensin 2 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human beta-Defensin 2 Assay. Assay range is 85.2-52,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: beta-Defensin 2
Defensins are members of a family of microbicidal and cytotoxic peptides produced by neutrophils. They are classified into alpha-, beta-, and theta-defensins based on their sizes and intramolecular disulfide bond patterns. Human beta-Defensin 2 (BD-2; aa 24 - 64) also known as defensin beta 2 (DEFB-2), is a secreted, 41 amino acid residue anti-microbial protein belonging to the defensin family. Mature defensins are cationic proteins with three intramolecular disulfides and a characteristic triple-stranded beta-sheet. Human beta-Defensin 2 expression is induced by inflammatory signals in multiple tissues, including airway epithelial cells and keratinocytes. At the amino acid sequence level, human beta-Defensin 2 shares 98% and 51% homology with the proteins from rhesus monkey and rat, respectively.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional beta-Defensin 2 Products
Product Documents for Simple Plex Discovery beta-Defensin 2 Assay
Product Specific Notices for Simple Plex Discovery beta-Defensin 2 Assay
For research use only