Simple Plex AAV9 Cartridge

Name: Simple Plex AAV9 Cartridge
Brand: ProteinSimple
SKU: SPCKB-OT-015059

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-OT-015059

Simple Plex AAV9 assay kit contains cartridge, sample diluent SD19, and wash buffer. Part # starting with ST01 ships in 2-3 days.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Bioprocess Samples (25 uL)
Sensitivity:	0.72 10^6 capsids/mL
Assay Range:	3.44 - 32,800 10^6 capsids/mL (Bioprocess Samples)
Simple Plex AAV9 Cartridge Summary:	Simple Plex assay is for the detection of Adeno-Associated Virus type 9 (AAV9) intact capsids in Bioprocess samples in Sample Diluent SD19.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex AAV9 Cartridge

Technical Data
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Product Specifications for Simple Plex AAV9 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Viral
Specificity	This assay recognizes intact AAV9 capsids.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Bioprocess Samples

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	34	34
Mean (10<sup>6</sup> capsids/mL)	295	13656	286	13670
Standard Deviation	10.2	357	16.4	654
CV%	3.5	2.6	5.7	4.8

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Bioprocess Samples (n=6)	95	90-104

Linearity

Samples containing and/or spiked with high concentrations of AAV9 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex AAV9 Cartridge

	Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex AAV9 Assay. Assay range is 3.44-32,800 10⁶ capsids/mL.

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: AAV9

Item	Value
Long Name	Adeno-associated Virus 9
Alternate Names	AAV9, Adeno-associated Virus 9, Coat protein, capsid, major coat protein

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Product Documents for Simple Plex AAV9 Cartridge

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Protocols & Manuals

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