Do the AAV assays recognize fully-assembled capsids?

Yes, these assays detect fully-assembled capsids. Non-assembled protein or partially assembled capsids will not be detected.

Do the AAV assays recognize both natural and recombinant AAV?

Yes, the AAV assays detect both natural and recombinant AAV.

Does the AAV8 assay recognize other AAV serotypes?

This assay recognizes intact AAV8 capsids. The AAV8 antibody (ADK8) used in this assay cross-reacts with AAV1, AAV3, AAV7, and AAVrh10.

Do the Simple Plex AAV assays distinguish between full and empty capsids?

No, the AAV assays do not distinguish between full and empty capsids. Complementary methods are required to distinguish full and empty capsids.

What diluent is provided with the AAV assays?

The AAV assays are supplied with SD19 concentrate. The concentrate should be diluted 1:5 in deionized or dilstilled water prior to use.

What is the minimum required dilution (MRD) for bio-process samples?

All samples require a minimum 2-fold dilution for use in Simple Plex assays, but further dilution may be needed for optimal performace. An appropriate dilution factor for each process matrix should be determined experimentally by assessment of sample linearity and spike recovery.

Simple Plex AAV8 Cartridge Best Seller

Name: Simple Plex AAV8 Cartridge
Brand: ProteinSimple
SKU: SPCKB-OT-007970

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-OT-007970

Product Datasheet / COA / SDS

Simple Plex AAV8 assay kit contains cartridge, sample diluent SD19, and wash buffer.

Key Product Details

Simple Plex AAV8 Cartridge Summary:

Simple Plex assay for the detection of Adeno-Associated Virus type 8 (AAV8) intact capsids in bioprocess samples.This assay uses PROGEN's AAV8 (ADK8) antibody.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Technical Data
Citations / FAQs

Product Specifications for Simple Plex AAV8 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Linearity

Samples containing and/or spiked with high concentrations of AAV8 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex AAV8 Cartridge

	AAV8 Simple Plex Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: AAV8

Item	Value
Long Name	Adeno-associated Virus 8
Alternate Names	AAV8

FAQs for Simple Plex AAV8 Cartridge

Compatible Instruments

Ella - Automated Immunoassay Platform

Ella Instrument. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex AAV8 Cartridge

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Simple Plex AAV8 Cartridge Best Seller

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-OT-007970

Key Product Details

Product Specifications for Simple Plex AAV8 Cartridge

Linearity

Scientific Data Examples for Simple Plex AAV8 Cartridge

Preparation and Storage

Background: AAV8

FAQs for Simple Plex AAV8 Cartridge

Compatible Instruments

Ella - Automated Immunoassay Platform

Product Documents for Simple Plex AAV8 Cartridge

Certificate of Analysis

CONTACT INFORMATION

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Simple Plex AAV8 Cartridge Best Seller

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-OT-007970

Key Product Details

Product Specifications for Simple Plex AAV8 Cartridge

Linearity

Scientific Data Examples for Simple Plex AAV8 Cartridge

Preparation and Storage

Background: AAV8

FAQs for Simple Plex AAV8 Cartridge

Compatible Instruments

Ella - Automated Immunoassay Platform

Product Documents for Simple Plex AAV8 Cartridge

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS