Non-Human Primate IL-15 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-011305
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Non-Human Primate IL-15 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 10 | 9 |
| Mean (pg/mL) | 10.3 | 562 | 10.8 | 540 |
| Standard Deviation | 0.666 | 40.2 | 0.598 | 29.6 |
| CV% | 6.5 | 7.2 | 5.6 | 5.5 |
Recovery for Non-Human Primate IL-15 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=3) | 112 | 108-114 |
| EDTA Plasma (n=4) | 107 | 98-114 |
| Heparin Plasma (n=4) | 106 | 105-106 |
| Serum (n=4) | 110 | 103-118 |
Linearity
Samples containing and/or spiked with high concentrations of IL-15 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Non-Human Primate IL-15 Cartridge
Non-Human Primate IL-15 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: IL-15
IL-15 (Interleukin 15) is a cytokine that induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells. It also induces dendritic cell differentiation and inflammatory activation, exhibits anti-tumor activity, and inhibits the deposition of lipid in adipocytes. Complexes of IL-15 with cell surface IL-15 R alpha interact with complexes of IL-2 R beta and the Common gamma Chain on adjacent cells. This transpresentation mechanism enables cells to respond to IL-15 even if they do not express IL-15 R alpha. Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells. The activity of circulating IL-15 is limited by its association with soluble IL-15 R alpha.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-15 Products
Product Documents for Non-Human Primate IL-15 Cartridge
Product Specific Notices for Non-Human Primate IL-15 Cartridge
For research use only