Mouse IgG Antibody
R&D Systems, part of Bio-Techne | Catalog # AF007
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse IgG
Specificity
Detects mouse IgG
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse IgG Antibody
Detection of IgG by Western Blot
Activin A regulation of MRTF-A contributes to alphaSMA induction by TGF beta1 in MC. a ActA (20 ng/ml) and TGF beta1 show an additive effect on alphaSMA promoter transcriptional activation at 24 h (n = 6). Activin inhibition with b follistatin (n = 7) and c an actA neutralizing antibody decrease TGF beta1-induced alphaSMA promoter transcriptional activity (n = 14–23). d actB neutralization does not decrease TGF beta1-induced alphaSMA promoter transcriptional activity (n = 6–9). e actA increases MRTF-A nuclear localization at 18 h (n = 5). TGF beta1-induced nuclear MRTF-A translocation is attenuated with f follistatin (n = 9) and g actA neutralization (n = 5). h ActA-induced alphaSMA promoter transcriptional activity is inhibited by MRTF-A siRNA (n = 9) and i the Smad3 inhibitor SIS3 (n = 3). *, **, ***, ****P < 0.05, 0.01, 0.001, 0.0001; one-way ANOVA with Tukey’s multiple comparisons post hoc test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36717814), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse IgG Antibody
Application
Recommended Usage
Western Blot
Use at a 1:5000 dilution in conjunction with an appropriate tertiary detection antibody.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Mouse IgG Antibody
Product Specific Notices for Mouse IgG Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...