Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4D2D9G8

Format

BSA Free

Concentration

1.0 mg/ml

View all IgG Heavy Chain Antibodies »

Product Specifications

Immunogen

Human IgG was isolated from human sera and purified by chromatography.

Specificity

Monoclonal anti-Human IgG is derived from a hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the IgG heavy chain, and has no cross reactivity with human IgM molecule as determined by an ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images

Western Blot: Mouse anti-Human IgG (H+L) Secondary Antibody (4D2D9G8) [NBP1-51523]

Western Blot: Mouse anti-Human IgG Heavy Chain Secondary Antibody (4D2D9G8) [NBP1-51523]

Western Blot: Mouse anti-Human IgG Heavy Chain Secondary Antibody (4D2D9G8) [NBP1-51523] - Western blot analysis using IgG mouse mAb (lane 1, 2) and Albumin mouse mAb (lane 3, 4) against human serum (lane 1, 3) and plasma (lane 2, 4).

Applications

Application
Recommended Usage

ELISA

1:10000

Western Blot

1:500 - 1:2000
Application Notes
This secondary antibody is useful for Western blot and ELISA.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgG Heavy Chain

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Heavy Chain Products

Product Documents

Product Datasheets

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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