Goat anti-Guinea Pig IgG Fc Secondary Antibody

Guinea Pig

ELISA, Immunoelectrophoresis

Unconjugated

Polyclonal Goat

LYOPH mg/ml

Highly purified normal IgG isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

The antiserum does not cross-react with any other component of the Guinea Pig immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail.

The reactivity of the antiserum is restricted to the Fc part of both subclasses IgG1 and IgG2. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal guinea pig serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No precipitation reaction is obtained with purified IgA, IgM, and IgG/Fab fragments.

Polyclonal

Goat

The lyophilized antiserum is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4C, not refrozen, and preferably used the same day.

Ion exchange chromatography

Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).

Whole antiserum

No Preservative

LYOPH mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.