Goat anti-Equine IgG F(ab')2 Secondary Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73473
Conjugate
Catalog #
Key Product Details
Species Reactivity
Equine
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Horse IgG F(ab')2 fragment
Specificity
No reaction was observed against Horse IgG F(c).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Horse IgG, Horse IgG F(ab')2 and Horse Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Horse IgG, Horse IgG F(ab')2 and Horse Serum
Applications for Goat anti-Equine IgG F(ab')2 Secondary Antibody [Biotin]
Application
Recommended Usage
ELISA
1:20000 - 1:100000
Immunohistochemistry
1:1000 - 1:5000
Western Blot
1:2000 - 1:10000
Application Notes
This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG F(ab')2
Additional IgG F(ab')2 Products
Product Documents for Goat anti-Equine IgG F(ab')2 Secondary Antibody [Biotin]
Product Specific Notices for Goat anti-Equine IgG F(ab')2 Secondary Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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