Rabbit anti-Canine IgG F(ab')2 Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73094
Key Product Details
Species Reactivity
Canine
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit Serum
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Dog IgG F(ab')2 fragment, rabbit anti Dog Fab2
Specificity
No reaction was observed against Dog IgG F(c).
Clonality
Polyclonal
Host
Rabbit
Isotype
Serum
Description
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Dog IgG, Dog IgG F(ab')2 and Dog Serum
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Applications for Rabbit anti-Canine IgG F(ab')2 Secondary Antibody
Application
Recommended Usage
ELISA
1:20000 - 1:100000
Immunohistochemistry
1:1000 - 1:5000
Western Blot
1:2000 - 1:10000
Application Notes
Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.
Formulation, Preparation, and Storage
Purification
Delipidation and Defibrination
Reconstitution
Reconstitute with 2.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG F(ab')2
Additional IgG F(ab')2 Products
Product Documents for Rabbit anti-Canine IgG F(ab')2 Secondary Antibody
Product Specific Notices for Rabbit anti-Canine IgG F(ab')2 Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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