Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody [DyLight 649] (Pre-adsorbed)

Human

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

DyLight 649 (Excitation = 654 nm, Emission = 673 nm)

Polyclonal Goat IgG

Pre-adsorbed

0.5 mg/ml

Human IgG-F(ab')2 Fragment

Minimum reactivity to mouse and rat.

Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with human F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with F(ab')2 from other species.

Polyclonal

Goat

IgG

Immunogen affinity purified

PBS containing 0.2% BSA

Pre-adsorbed

0.09% Sodium Azide

0.5 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C. Do not freeze.