Key Product Details

Species Reactivity

Human

Applications

Dot Blot, ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgA Heavy Chain Secondary Antibodies »

Product Specifications

Immunogen

Human IgA alpha heavy chain

Specificity

No reaction was observed against other human heavy or light chain proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose followed by solid phase adsorption(s). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgA and Human Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Rabbit anti-Human IgA Heavy Chain Secondary Antibody

Western Blot: Rabbit anti-Human IgA Heavy Chain Secondary Antibody [NBP1-72777]

Western Blot: Rabbit anti-Human IgA Heavy Chain Secondary Antibody [NBP1-72777]

Western Blot: Rabbit anti-Human IgA Secondary Antibody (Pre-absorbed) [NBP1-72777] - Lane 1: Human IgA Load: 100 ng per lane Primary antibody: HUMAN IgA (alpha chain) Antibody at 1:1000 for 60 min at RT Secondary antibody: DyLight 649 goat anti-rabbit at 1:20,000 for 30 min at RT Block: 5% BLOTTO 30 min at RT Predicted/Observed size: 60and 28 kDa, 60 and 28 kDa
Dot Blot: Rabbit anti-Human IgA Heavy Chain Secondary Antibody [NBP1-72777] - Antigen: Human IgA. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Incubated for 1hr at RT; blocked for 60 min at RT. Image from the FITC version of this antibody.

Applications for Rabbit anti-Human IgA Heavy Chain Secondary Antibody

Application
Recommended Usage

ELISA

1:5000 - 1:20000

Immunohistochemistry

1:250 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This product is tested for ELISA and western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Rabbit anti-Human IgA Heavy Chain Secondary Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rabbit anti-Human IgA Heavy Chain Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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