What is the heparin binding activity of FGF and VEGF?

Name: Recombinant Rat VEGF 164 Protein
Brand: Novus Biologicals
SKU: NBP2-35310

These proteins are not assayed for their ability to bind Heparin. More information about the FGF family of growth factors is available in this review article: Basilico, C. (1992) Adv. Can. Res. 59:115.

Recombinant Rat VEGF 164 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35310

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Key Product Details

Source

E. coli

Accession #

P16612

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all VEGF 164 Proteins and Enzymes »

Product Specifications

Description

A disulfide-linked homodimeric protein, consisting of two 165 amino acid polypeptide chains with Met at N-terminus corresponding to VEGF 164 Source: E. coli

Uniprot ID: P16612

Amino Acid Sequence: MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of VEGF 164 as determined by LAL method.

Predicted Molecular Mass

38.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

VEGF 165 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat VEGF 164 Protein

SDS-PAGE: Recombinant Rat VEGF 164 Protein [NBP2-35310]

SDS-Page: Rat VEGF 164 Protein [NBP2-35310]

Formulation, Preparation and Storage

NBP2-35310

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.8.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: VEGF 164

Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells, which stimulates vasculogenesis and angiogenesis. VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. Mouse and rat express alternately spliced isoforms of 120, 164, and 188 amino acids (a.a.) in length. Recombinant Rat VEGF164 contains 165 amino acids residues and it is a disulfide-linked homodimer. In addition, it shares 97 % a.a. sequence identity with corresponding regions of mouse, 88 % with human and bovine, 89 % with porcine and canine, and 90 % with feline and equine VEGF, respectively.

Gene Symbol

VEGFA

UniProt

P16612 (Rat)

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COA

Product Specific Notices for Recombinant Rat VEGF 164 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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