Recombinant E. coli Thioredoxin Reductase 1/TRXR1 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44981

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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all Thioredoxin Reductase 1/TRXR1 Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-321 of E.coli Thioredoxin Reductase 1/TRXR1

Source: E.coli

Amino Acid Sequence: MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

34.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 10 units/mg, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412nm.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli Thioredoxin Reductase 1/TRXR1 Protein

SDS-PAGE: Recombinant E. coli Thioredoxin Reductase 1/TRXR1 Protein [NBP1-44981]

SDS-PAGE: Recombinant E. coli Thioredoxin Reductase 1/TRXR1 Protein [NBP1-44981]

SDS-Page: thioredoxin reductase 1 Protein [NBP1-44981] - TRXB, 34.6 kDa (321aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-44981
Formulation 20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thioredoxin Reductase 1/TRXR1

TRXB(Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant E.coli TRXB protein was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

GRIM-12, KDRF, TR1, TRXR1, TRXRD1, TXNR, TXNRD1

Gene Symbol

TXNRD1

Additional Thioredoxin Reductase 1/TRXR1 Products

Product Documents for Recombinant E. coli Thioredoxin Reductase 1/TRXR1 Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant E. coli Thioredoxin Reductase 1/TRXR1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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