Recombinant Human SOX2 TAT Protein

Name: Recombinant Human SOX2 TAT Protein
Brand: Novus Biologicals
SKU: NBP2-35268

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35268

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Product Specifications
Scientific Data
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Background
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Key Product Details

Source

E. coli

Accession #

P48431

Conjugate

Unconjugated

Applications

SDS-PAGE

View all SOX2 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 330 amino acids, including the 317 residues of full-length SOX2 and a 13-residue C-terminal TAT peptide (GGYGRKKRRQRRR) Source: E. coli

Uniprot ID: P48431

Amino Acid Sequence: MYNMMETELK PPGPQQTSGG GGGNSTAAAA GGNQKNSPDR VKRPMNAFMV WSRGQRRKMA QENPKMHNSE ISKRLGAEWK LLSETEKRPF IDEAKRLRAL HMKEHPDYKY RPRRKTKTLM KKDKYTLPGG LLAPGGNSMA SGVGVGAGLG AGVNQRMDSY AHMNGWSNGS YSMMQDQLGY PQHPGLNAHG AAQMQPMHRY DVSALQYNSM TSSQTYMNGS PTYSMSYSQQ GTPGMALGSM GSVVKSEASS SPPVVTSSSH SRAPCQAGDL RDMISMYLPG AEVPEPAAPS RLHMSQHYQS GPVPGTAING TLPLSHMGGY GRKKRRQRRR

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of SOX2 as determined by LAL method.

Predicted Molecular Mass

36.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SOX2 TAT Protein

SDS-PAGE: Recombinant Human SOX2 TAT Protein [NBP2-35268]

SDS-Page: Human SOX2 TAT Protein [NBP2-35268]

Formulation, Preparation and Storage

NBP2-35268

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4, with 5% Trehalose.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: SOX2

Sox2, also named SRY (sex determining region Y)-box 2, is belonging to the Sox family and it is encoded by the Sox gene in human. This protein family shares highly conserved DNA binding domains known as HMG (High-mobility group) box domains containing approximately 80 amino acids. Sox2 plays a role in maintenance of embryonic and neural stem cells and holds great promise in research involving induced pluripotency, an emerging and very promising field of regenerative medicine. Mature human Sox2 shares 100 amino acid sequence identity with murine and rat Sox2. Recombinant human Sox2-TAT expressed in E. coli is a 36 kDa protein containing 330 amino-acid residues, including the 317 residues of full-length Sox2 and a 13-residue Cterminal TAT peptide (GGYGRKKRRQRRR).

Long Name

Transcription Factor SOX2

Alternate Names

ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2

Gene Symbol

SOX2

UniProt