A recombinant protein with a His (N-Term) tag corresponding to the amino acids Ala1-Gly638 of SARS-CoV-2 nsp2

Source: E.coli

Accession: YP_009725298.1

Purity

>90%, by SDS-PAGE quantitative densitometry by Coomassie Blue Staining

Predicted Molecular Mass

72.81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant SARS-CoV-2 nsp2 His (N-Term) Protein

SDS-PAGE: Recombinant SARS-CoV-2 nsp2 His (N-Term) Protein [NBP3-07083]

SDS-Page: Recombinant SARS-CoV-2 nsp2 His (N-Term) Protein [NBP3-07083] - SDS-PAGE Image

Formulation, Preparation and Storage

NBP3-07083

Formulation	PBS, pH 7.5
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 nsp2

SARS-CoV-2 Nonstructural Protein 2 (NSP2) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP2 is 638 amino acids (aa) with a theoretical molecular weight of 70.5 kDa (1,2). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP2 has 68.3% sequence identity and 90% sequence similarity (1). SARS-CoV-2 NSP2 is shown to bind two host proteins, prohibitin 1 (PHB1) and prohibitin 2 (PHB2) (1). The NSP2-PHB protein interaction indicates that SARS-CoV-2 NBP2 might function in disturbing the host-cell environment during viral infection (1). A SARS-CoV-2 interactome study revealed that NSP2 has a role in vesicle trafficking, specifically reconfiguring endoplasmic reticulum and Golgi trafficking during viral infection via the WASH complex (Wiskott Aldrich Syndrome protein and scar homologue complex) (2).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

Alternate Names

Non-structural protein 2, NSP2, p65 homolog

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp2 Products

Product Documents for Recombinant SARS-CoV-2 nsp2 His (N-Term) Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant SARS-CoV-2 nsp2 His (N-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Recombinant SARS-CoV-2 nsp2 His (N-Term) Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07083

Key Product Details

Tag

Conjugate

Applications

View all SARS-CoV-2 nsp2 Proteins and Enzymes »

Product Specifications

Description