Recombinant Mouse Mast Cell Protease-6/Mcpt6 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3736-SE
Key Product Details
- R&D Systems NS0-derived Recombinant Mouse Mast Cell Protease-6/Mcpt6 Protein (3736-SE)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Mast Cell Protease-6/Mcpt6 protein
Ala22-Ser276, with a C-terminal 10-His tag
Ala22-Ser276, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala22
Predicted Molecular Mass
30 kDa
SDS-PAGE
37 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >500 pmol/min/µg, as measured under the described conditions.
The specific activity is >500 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 5 using 3736-SE in the following applications:
Formulation, Preparation and Storage
3736-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Mast Cell Protease-6/Mcpt6
Tryptases are trypsin-like serine proteases, and beta tryptases appear to be the main isoenzymes expressed in mast cells (1). Mast Cell Protease-6 (MCP-6, Mcpt6, or Tpsb2) is one of beta tryptases produced from mouse mast cells. It is stored in secretory granules of mast cells, where it forms active tetramers with heparin proteoglycan. Because of the unique arrangement of the active sites in the tetramer, which are facing a narrow central pore, MCP-6 is resistant to macromolecular protease inhibitors (2). When mast cells are activated, MCP-6 is released along with other proteins in secretory granules, participating in provoking inflammatory conditions (3). MCP-6 has been implicated as a mediator in the pathogenesis of asthma and other allergic disorders in mouse models.
References
- Caughey, G. H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1535 .
- Sommerhoff, C.P. et al. (1999) Proc. Natl. Acad. Sci. USA. 96:10984.
- Hallgren, J. and G. Pejler, (2006) FEBS J. 273:1871.
Alternate Names
Mast Cell Protease6, Mcpt6
Gene Symbol
TPSAB1
UniProt
Additional Mast Cell Protease-6/Mcpt6 Products
Product Documents for Recombinant Mouse Mast Cell Protease-6/Mcpt6 Protein, CF
Product Specific Notices for Recombinant Mouse Mast Cell Protease-6/Mcpt6 Protein, CF
For research use only
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