Recombinant Mouse Integrin alpha 10 beta 1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7827-AB

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
7827-AB-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse Integrin alpha 10 beta 1 Protein (7827-AB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession #

NP_001289400.1 (Integrin alpha10) & P09055 (Integrin beta1)

Structure / Form

Noncovalently-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Integrin alpha 10 beta 1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 10 beta 1 protein
Mouse Integrin alpha10
Phe23-Thr1118 (Lys ins between Gln858 and Ala859)
Accession # NP_001289400.1		 His-Pro GGGSGGGS Acidic Tail HHHHHH
Mouse Integrin beta1
(Gln21-Asp728)
Accession # P09055		 His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe23 (Integrin  alpha10) & Gln21 predicted: No results obtained, sequencing might be blocked (Integrin beta1)

Predicted Molecular Mass

129.2 kDa (Integrin  alpha10) & 86.5 kDa (Integrin beta1)

SDS-PAGE

125-140 kDa & 155-170 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Mouse Laminin I (Catalog # 3400-010-01) is coated at 10 μg/mL, Recombinant Mouse Integrin  alpha10 beta1 binds with an apparent Kd <10 nM.

Formulation, Preparation and Storage

7827-AB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 300 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 10 beta 1

Integrin alpha10 beta1 is one of twelve integrin family adhesion receptors that share the beta1 subunit (1‑3). The non‑covalent heterodimer of 160 kDa alpha11 and 130 kDa beta1/CD29 type I transmembrane glycoprotein subunits is expressed mainly on chondrocytes within cartilage, but also in fibrous connective tissues such as heart valves and ligaments (3, 4). The alpha10 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3, 5). The beta1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside‑out) signaling convert it to its most active, extended and open conformation (1, 2). The 1100 amino acid (aa) mouse  alpha10 extracellular domain (ECD) shares 96% aa sequence identity with  rat and 88‑89% with human, rabbit, porcine, canine and bovine alpha10, while the 708 aa mouse beta1 ECD shares 98% aa identity with rat and 93‑94% with human, bovine, porcine, ovine, canine and feline beta1. A potential mouse alpha10 splice variant diverges at aa 1039 and is terminated prematurely. If translated, this variant would result in a secreted protein (6). I domain‑containing beta1 integrins alpha1 beta1, alpha2 beta1, alpha10 beta1 and alpha11 beta1 all bind collagens; all but alpha11 beta1 also bind laminins (5, 7, 8). During cartilage differentiation, alpha10 beta1 is thought to be the main integrin binding type II and IX cartilage collagens (3‑5, 7‑10). However, deletion of mouse alpha10 causes a mild phenotype including slightly shortened bones and narrowed hypertrophic zones, indicating that another collagen‑binding integrin, likely alpha2 beta1, may compensate for alpha10 beta1 functions (11). Migration of melanoma cells has been noted to correlate with alpha10 beta1 expression (12).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Camper, L. et al. (1998) J. Biol. Chem. 273:20383.
  4. Camper, L. et al. (2001) Cell Tiss. Res. 306:107.
  5. Tulla, M. et al. (2001) J. Biol. Chem. 276:48206.
  6. Bengtsson, T. et al. (2001) Matrix Biol. 20:565.
  7. McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
  8. Popova, S.N. et al. (2007) Acta Physiol. 190:179.
  9. Varas, L. et al. (2007) Stem Cells Dev. 16:965.
  10. Gigout, A. et al. (2008) J. Biol. Chem. 283:31522.
  11. Bengtsson, T. et al. (2004) J. Cell Sci. 118:939.
  12. Wenke, A.K. et al. (2007) Cell Oncol. 29:373.

UniProt

NP_001289400.1 (Integrin alpha10) & P09055 (Integrin beta1)

Additional Integrin alpha 10 beta 1 Products

Product Documents for Recombinant Mouse Integrin alpha 10 beta 1 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse Integrin alpha 10 beta 1 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.