Recombinant Mouse alpha3GalT/GGTA1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7887-GT

R&D Systems, part of Bio-Techne
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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse alpha3GalT/GGTA1 Protein (7887-GT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession #

P23336

Conjugate

Unconjugated

Applications

Enzyme Activity

View all alpha3GalT/GGTA1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse alpha3GalT/GGTA1 protein
Trp80-Val394, with C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp80

Predicted Molecular Mass

38 kDa

SDS-PAGE

35-43 kDa, reducing conditions

Activity

Measured by its ability to transfer galactose from UDP-galactose to alpha-Lactose.
The specific activity is >850 pmol/min/μg, as measure under the described conditions.

Formulation, Preparation and Storage

7887-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Brij-35.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: alpha3GalT/GGTA1

GGTA1, also designated as alpha3GalT, is an alpha-1,3 galactosyltransferase that catalyzes the transfer of galactose from UDP-alpha -D-galactose to various glycoconjugates to form non‑reducing terminal alpha-1,3-linked galactosyl moieties (1). This enzyme is expressed in many mammalian species but is absent from humans, apes, and Old World monkeys due to the mutational inactivation of the gene (2). Therefore, humans do not have galactosyl alpha-1,3-galactosyl structures on glycoconjugates and produce large amounts of antibody to this alpha-Gal epitope. The production of this endogenous antibody results from exposure to intestinal bacteria and other antigens (3). GGTA1 is of medical interest because the immune responses may be enhanced when selected targets are decorated with galactosyl alpha-1,3-galactosyl structures (4). Additionally, the presence of anti-alpha -Gal antibodies is a barrier to xenotransplantation of organs, therefore, genetic manipulation of the GGTA1 gene and the development of inhibitors of the enzyme are of interest as complementary approaches to reduce rejection of xenotransplanted organs. Structurally and mechanistically GGTA1 is a model for several homologous glycosyltransferases that differ in donor and acceptor substrate specificity, including the histo blood group A and B glycosyltransferases, Forssman glycolipid synthase, and isogloboside 3 synthase (5). The activity of the recombinant GGTA1 is measured using a phosphatase-coupled glycosyltransferase assay (6).

References

  1. Larsen RD, et al.(1989) Proc. Natl. Acad. Sci. U. S. A. 86:8227.
  2. Joziasse, D.H. et al. (1992) J. Biol. Chem. 267:5534.
  3. Wigglesworth KM, et al. (2011) J. Immunol. 186:4422.
  4. Dequchi, T. et al. (2010) Cancer Res. 70:5259.
  5. Breton, C. et al. (2005) Glycobiology 16:29R.
  6. Wu, Z.L. et al. (2011) Glycobiology. 21:727.

Long Name

alpha 1,3-Galactosyltransferase

Alternate Names

alpha Gal, GALT, GGTA1, Glt6d1

Entrez Gene IDs

14594 (Mouse); 246766 (Rat); 396733 (Porcine)

Gene Symbol

GGTA1

UniProt

P23336

Additional alpha3GalT/GGTA1 Products

Product Documents for Recombinant Mouse alpha3GalT/GGTA1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse alpha3GalT/GGTA1 Protein, CF

For research use only

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