Recombinant Human MINDY3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E-621

R&D Systems, part of Bio-Techne
Discontinued Product
E-621 has been discontinued. View all MINDY3 products.

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

View all MINDY3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human MINDY3 protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

50 kDa

Activity

Recombinant Human MINDY-3 is a Ubiquitin-specific deconjugating enzyme that cleaves K48-linked polyubiquitin chains.

Reviewed Applications

Read 1 review rated 5 using E-621 in the following applications:

Formulation, Preparation and Storage

E-621
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: MINDY3

MINDY-3/FAM188A is a member of a newly characterized cysteine protease family of deubiquitinases ("DUBs").  In humans, the MINDY (MIU containing novel DUB family) family has at least four members, with an additional three being MINDY-1/FAM63A, MINDY-2/FAM63B and MINDY-4/FAM188B.  The catalytic domain of MINDY DUBs adopts a conformation that is distinct from other known DUB classes, and these enzymes are highly selective at cleaving K48-linked polyubiquitin chains.  With a predicted molecular weight of 50 kDa, human MINDY-3 is 95% identical to both its murine and rat orthologs.  This recombinant protein is untagged.

References

  1. Abdul-Rehman S.A. et al. (2016)  Mol. Cell 63: 146
  2. Kristariyanto Y.A. et al. (2017) EMBO Reports  DOI 10.15252/embr.201643205

Long Name

Ubiquitin Carboxyl-Terminal Hydrolase MINDY-3

Alternate Names

C10orf97, FAM188A, MSTP126, My042

Entrez Gene IDs

80013 (Human)

Gene Symbol

MINDY3

Additional MINDY3 Products

Product Documents for Recombinant Human MINDY3 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human MINDY3 Protein, CF

For research use only

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