Recombinant Human LILRB4/CD85k/ILT3 Fc Biotin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT10429

Fc Chimera Biotinylated
R&D Systems, part of Bio-Techne
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BT10429-050
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human LILRB4/CD85k/ILT3 Fc Biotin Protein (BT10429)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Structure / Form

Biotinylation via amines

Conjugate

Biotin

Applications

Bioactivity

View all LILRB4/CD85k/ILT3 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LILRB4/CD85k/ILT3 protein
Human LILRB4
(Gly24-Glu259)
Accession # NP_001265355.2		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

53 kDa

SDS-PAGE

50-70 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera binds to Human Fibronectin Protein (Catalog # 1918-FN) with an ED50 of 0.400-4.00 μg/mL.

Scientific Data Images for Recombinant Human LILRB4/CD85k/ILT3 Fc Biotin Protein, CF

Biotinylated Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera Protein (Catalog # BT10429) binds to Human Fibronectin Protein (1918-FN) with an ED50 of 0.400-4.00 μg/mL.

Biotinylated Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human LILRB4/CD85k/ILT3 Fc Chimera Protein (Catalog # BT10429) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa and 100-140 kDa, respectively.

Formulation, Preparation and Storage

BT10429
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRB4/CD85k/ILT3

ILT3, also known as LILRB4, CD85k, and LIR5, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation (1). Mature human ILT3 consists of an extracellular domain (ECD) with two Ig-like domains, a transmembrane segment, and a cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM) (2). The mature ECD of human ILT3 shares 44% amino acid identity with mouse ILT3. Alternative splicing of human ILT3 generates an isoform that lacks the first ITIM and a secreted isoform that circulates in the serum of cancer patients (3, 4). ILT3 is expressed on dendritic cells (DC), monocytes, macrophages, and vascular endothelial cells (EC) (2, 5, 6). Ligation of ILT3 triggers ITIM-mediated inhibition of cell-activating signaling, leading to enhanced immune tolerance and reduced allogeneic graft rejection (2, 4, 7, 8). Soluble ILT3 induces the differentiation of CD8+ T suppressor cells (Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL (4, 7, 9). In turn, CD8+ Ts cells induce ILT3 up-regulation and a tolerogenic phenotype in monocytes, DC, and EC (5, 6, 8, 10, 11). Recently, a novel anti-LILRB4 CAR-T Cell was been used to treat monocytic acute myeloid leukemia in humanized hematopoietic-reconstituted mice models (12).

References

  1. Vlad, G. et al. (2010) Int. Rev. Immunol. 29:119.
  2. Cella, M. et al. (1997) J. Exp. Med. 185:1743.
  3. Heinzmann, A. et al. (2000) Eur. J. Immunogenet. 27:121.
  4. Suciu-Foca, N. et al. (2007) J. Immunol. 178:7432.
  5. Gleissner, C.A. et al. (2007) Eur. J. Immunol. 37:177.
  6. Manavalan, J.S. et al. (2004) Int. Immunol. 16:1055.
  7. Vlad, G. and N. Suciu-Foca (2012) Exp. Mol. Pathol. 93:294.
  8. Chang, C.C. et al. (2002) Nat. Immunol. 3:237.
  9. Vlad, G. et al. (2006) Int. Immunopharmacol. 6:1889.
  10. Manavalan, J.S. et al. (2003) Transpl. Immunol. 11:245.
  11. Brenk, M. et al. (2009) J. Immunol. 183:145.
  12. John, S. et al. (2018) Mol. Ther. 26:2487.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4

Alternate Names

CD85k, HM18, ILT3, LIR5

Entrez Gene IDs

11006 (Human); 14728 (Mouse); 107128256 (Cynomolgus Monkey)

Gene Symbol

LILRB4

Additional LILRB4/CD85k/ILT3 Products

Product Documents for Recombinant Human LILRB4/CD85k/ILT3 Fc Biotin Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human LILRB4/CD85k/ILT3 Fc Biotin Protein, CF

For research use only

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