Recombinant Human Integrin alpha 6 beta 1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7809-A6

R&D Systems, part of Bio-Techne
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7809-A6-050
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Integrin alpha 6 beta 1 Protein (7809-A6)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession #

NP_000201 ( alpha6 subunit) & P05556 ( beta1 subunit)

Structure / Form

Noncovalently-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Integrin alpha 6 beta 1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 6 beta 1 protein
Human Integrin alpha6
(Phe24-Ser1012)
Accession # NP_000201		 GGGSGGGS Acidic Tail HHHHHH
Human Integrin beta1
(Gln21-Asp728)
Accession # P05556		 GGGSGGGS Basic Tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe24 ( alpha6 subunit) & Gln21 predicted: No results obtained, sequencing might be blocked ( beta1 subunit)

Predicted Molecular Mass

119 kDa ( alpha6 subunit) & 86.4 kDa ( beta1 subunit)

SDS-PAGE

110-140 kDa & 140 - 170 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Laminin alpha4 (Catalog # 7340-A4) is coated at 5 μg/mL, Recombinant Human Integrin  alpha6 beta1 binds with apparent KD <2nM.

Reviewed Applications

Read 2 reviews rated 5 using 7809-A6 in the following applications:

Formulation, Preparation and Storage

7809-A6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 400 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 6 beta 1

Integrin alpha6 beta1, also called platelet glycoprotein GPIc-IIa, is a laminin binding integrin that is expressed on T cells, monocytes, endothelial cells, stem cells, and platelets (1-9). The non-covalent heterodimer is composed of ~150 kDa alpha6/CD49f and 130 kDa beta1/CD29 type I transmembrane glycoprotein subunits (2). While alpha6 pairs only with beta1 or beta4, twelve integrins share the beta1 subunit (1-5). The alpha6 subunit is cleaved into extracellular heavy and transmembrane light chains (3). Alternative splicing in the human alpha6 extracellular domain (ECD) at amino acid (aa) 216 creates X1 (ubiquitous), X2 and X1X2 isoforms, while splicing at a mouse or human cytoplasmic site creates A and B isoforms (10, 11). These forms do not appear to alter the binding specificity (4, 10, 11). The beta1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The human alpha6 (X1) heavy chain shares 94‑95% aa identity with mouse, rat, bovine, and canine alpha6, and the human beta1 ECD shares 92‑96% aa sequence identity with rat, bovine, mouse, and feline beta1. alpha6 beta1 shows broad specificity for adhesion to laminin isoforms (4, 10). Its expression on human and mouse pluripotent stem cells is important for attachment, expansion, and self‑renewal on LN‑511 (laminin  alpha5  beta1 gamma1) (6, 7). The secreted protein Netrin-4 and the laminin  gamma1 subunit form an adhesion‑activating complex with alpha6 beta1 on mouse neural stem cells and human lymphatic endothelial cells that promotes lymphangiogenesis (8, 9). alpha6 beta1 up‑regulation on cancers such as prostate, glioma, and hepatoma is reported to enhance tumorigenicity, motility, invasion and metastasis (12‑14). alpha6 beta1 cleavage via uPA (urokinase-type plasminogen activator) facilitates tumorigenicity in prostate cancers, and interaction of hepatoma alpha6 beta1 with EMMPRIN/CD147 may also enhance tumorigenicity by inducing uPA and other metalloproteinases (12, 13).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Tamura, R.N. et al. (1990) J. Cell Biol. 111:1593.
  4. Nishiuchi, R. et al. (2006) Matrix Biol. 25:189.
  5. Sonnenberg, A. and C.J.T. Linders (1990) J. Cell Science 96:207.
  6. Rodin, S. et al. (2010) Nat. Biotech. 28:611.
  7. Domogatskaya A. et al. (2008) Stem Cells 26:2800.
  8. Staquicini, F.I. et al. (2009) Proc. Natl. Acad. Sci. USA 106:2903.
  9. Larrieu-Lahargue, F. et al. (2011) Circ. Res. 109:770.
  10. Delwel, G. O. et al. (1995) Cell Adhes. Commun. 3:143.
  11. Hogervorst, F. et al. (1993) J. Cell Biol. 121:179.
  12. Sroka, I.C. et al. (2011) Mol. Cancer Res. 9:1319.
  13. Dai, J.Y. et al. (2009) BMC Cancer 9:337.
  14. Delamarre, E. et al. (2009) Am. J. Pathol. 175:844.

Alternate Names

CD49f, ITGA6B, VLA-6

UniProt

NP_000201 ( alpha6 subunit) & P05556 ( beta1 subunit)

Additional Integrin alpha 6 beta 1 Products

Product Documents for Recombinant Human Integrin alpha 6 beta 1 Protein, CF

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