Recombinant Human IL-4R alpha Protein

R&D Systems, part of Bio-Techne | Catalog # 230-4R

R&D Systems, part of Bio-Techne
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Carrier Free
230-4R-025/CF
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With Carrier
230-4R-025
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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human IL-4R alpha Protein (230-4R)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession #

P24394

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-4R alpha Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-4 R alpha protein
Gly24-His232

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

24 kDa

SDS-PAGE

30-35 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-4-dependent proliferation of TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
Approximately 5-25 ng/mL of IL-4 R alpha will inhibit 50% of the biological response due to 0.2 ng/mL of recombinant human IL-4.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 230-4R
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 230-4R/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4R alpha

Interleukin 4 Receptor alpha (IL-4 R alpha), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. IL-4 R alpha plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis (1 - 5). Mature human IL-4 R alpha consists of a 207 amino acid (aa) extracellular domain (ECD) that contains a cytokine binding region and one fibronectin type-III domain, a 24 aa transmembrane segment, and a 569 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif (6, 7). Within the ECD, human IL-4 Ra shares 51% aa sequence identity with mouse and rat IL-4 R alpha. Soluble forms of IL-4 R alpha, generated by alternate splicing or proteolysis, retain ligand binding properties and inhibit IL-4 bioactivity (8 - 11). IL-4 R alpha is a component of two distinct receptor complexes and shows species selectivity between human and mouse (6). It can associate with the common gamma chain ( gammac) to form the IL-4 responsive type I receptor in which gammac increases the affinity for IL-4 and enables signaling (12, 13). It can alternatively associate with IL-13 R alpha1 to form the type II receptor which is responsive to both IL-4 and IL-13 (14, 15). The use of shared receptor components contributes to the overlapping biological effects of IL-4 and IL‑13 as well as other cytokines that utilize gammac (i.e. IL-2, IL-7, IL-9, IL-15, and IL-21) (16, 17).

References

  1. Wills-Karp, M. and F.D. Finkelman (2008) Sci. Signal. 1:pe55.
  2. Gordon, S. and F.O. Martinez (2010) Immunity 32:593.
  3. Kuperman, D.A. and R.P. Schleimer (2008) Curr. Mol. Med. 8:384.
  4. Li, Z. et al. (2009) Cell. Mol. Immunol. 6:415.
  5. Lee, Y.W. et al. (2010) Biomol. Ther. 18:135.
  6. Idzerda, R.L. et al. (1990) J. Exp. Med. 171:861.
  7. Galizzi, J.P. et al. (1990) Int. Immunol. 2:669.
  8. Kruse, S. et al. (1999) Int. Immunol. 11:1965.
  9. Blum, H. et al. (1996) J. Immunol. 157:1846.
  10. Jung, T. et al. (1999) Int. Arch. Allergy Immunol. 119:23.
  11. Mosley, B. et al. (1989) Cell 59:335.
  12. Kondo, M. et al. (1993) Science 262:1874.
  13. Russell, S.M. et al. (1993) Science 262:1880.
  14. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. 93:497.
  15. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
  16. Ramalingam, T.R. et al. (2008) Nat. Immunol. 9:25.
  17. Overwijk, W.W. and K.S. Schluns (2009) Clin. Immunol. 132:153.

Long Name

Interleukin 4 Receptor alpha

Alternate Names

CD124, IL-4 R alpha, IL-4Ra, IL4R, IL4R alpha

Entrez Gene IDs

3566 (Human); 16190 (Mouse); 102133649 (Cynomolgus Monkey)

Gene Symbol

IL4R

UniProt

P24394 (Human)

Additional IL-4R alpha Products

Product Documents for Recombinant Human IL-4R alpha Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 230-4R (with carrier)
Download SDS 230-4R/CF (carrier free)

Product Specific Notices for Recombinant Human IL-4R alpha Protein

For research use only

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