Recombinant Human IL-21 GMP Protein, CF GMP

R&D Systems, part of Bio-Techne | Catalog # 8879-GMP

Animal-Free
R&D Systems, part of Bio-Techne
Discontinued Product
8879-GMP has been discontinued. An alternative/replacement product is available: BT-021-GMP. View all IL-21 products.

Key Product Details

Source

E. coli

Accession #

Q9HBE4.3

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-21 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-21 protein
Gln32-Ser162, with a N-terminal Met
Produced in an animal-free laboratory. Manufactured and tested under cGMP guidelines

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met-Gln-Asp-Arg-His-Met-Ile-Arg-Met-Arg

Predicted Molecular Mass

15 kDa

SDS-PAGE

16 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using B9 mouse hybridoma cells.

The ED50 for this effect is 5.00-50.0 ng/mL.

The specific activity of recombinant human IL-21 is >1.00 x 106 units/mg, which is calibrated against an internal reference standard for human IL-21.



Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells.
The ED50 for this effect is ≤8 ng/mL.

Host Cell Protein

< 0.1 ng per μg of protein when tested by ELISA.

Mycoplasma

Negative for Mycoplasma.

Scientific Data Images for Recombinant Human IL-21 GMP Protein, CF

Recombinant Human IL‑21 GMP Protein Bioactivity.

Recombinant Human IL‑21 GMP (Catalog # 8879-GMP) induces B9 mouse hybridoma cell proliferation. The ED50 for this effect is 5.00-50.0 ng/mL
Recombinant Human IL-21 GMP Protein Bioactivity

Recombinant Human IL-21 GMP Protein Bioactivity

GMP-grade Recombinant Human IL-21 (Catalog # 8879‑GMP) enhances IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is ≤8 ng/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of GMP IL-21.
Recombinant Human IL-21 GMP Protein SDS-PAGE

Recombinant Human IL-21 GMP Protein SDS-PAGE

2 μg/lane of GMP-grade Recombinant Human IL-21 (Catalog # 8879-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing R band at 16.4 kDa and NR band at 14.8 kDa, respectively.

Formulation, Preparation and Storage

8879-GMP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100-200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: IL-21

Interleukin-21 (IL-21) is an approximately 14 kDa four-helix-bundle member of the family of cytokines that utilize the common gamma chain ( gammac) as a receptor subunit. gammac is also a subunit of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15 (1). IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells (2-6). It exerts its biological effects through a heterodimeric receptor complex of gammac and the IL-21-specific IL-21 R (2, 7). Tfh-derived IL-21 plays an important role in the development of humoral immunity through its autocrine effects on the Tfh cell and paracrine effects on immunoglobulin affinity maturation, plasma cell differentiation, and B cell memory responses (4, 8, 9). It is also required for the migration of dendritic cells to draining lymph nodes (10). IL-21 regulates several aspects of T cell function. It co-stimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization (3, 5, 6, 11, 12). It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells (13, 14). IL-21 R engagement enhances the cytolytic activity and IFN-gamma production of activated NK cells but limits the expansion of resting NK cells (15). In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation (16). Dysregulation of the IL-21/IL-21 R system contributes to the development of multiple immunological disorders (1, 17). The 133 amino acid (aa) mature human IL-21 shares 63% and 61% aa sequence identity with mouse and rat IL-21, respectively. Alternative splicing generates an additional isoform with a substitution of the C-terminal 16 amino acids (18).

References

  1. Tangye, S.G. (2015) Curr. Opin. Immunol. 34:107.
  2. Parrish-Novak, et al. (2000) Nature 408:57.
  3. Coquet, J.M. et al. (2007) J. Immunol. 178:2827.
  4. Vogelzang, A. et al. (2008) Immunity 29:127.
  5. Korn, T. et al. (2007) Nature 448:484.
  6. Nurieva, R. et al. (2007) Nature 448:480.
  7. Asao, H. et al. (2001) J. Immunol. 167:1.
  8. Zotos, D. et al. (2010) J. Exp. Med. 207:365.
  9. Rankin, A.L. et al. (2011) J. Immunol. 186:667.
  10. Jin, H. et al. (2009) J. Clin. Invest. 119:47.
  11. Frohlich, A. et al. (2009) Science 324:1576.
  12. Yi, J.S., et al. (2009) Science 324:1572.
  13. Peluso, I. et al. (2007) J. Immunol. 178:732.
  14. Bucher, C. et al. (2009) Blood 114:5375.
  15. Kasaian, M.T. et al. (2002) Immunity 16:559.
  16. Tamagawa-Mineoka, R. et al. (2011) J. Invest. Dermatol. 131:1513.
  17. Ma, J. et al. (2011) Cytokine 56:133.
  18. Rahman, M. et al. (2007) FEBS Lett. 581:4001.

Long Name

Interleukin 21

Alternate Names

CVID11, IL21, Za11

Entrez Gene IDs

59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit)

Gene Symbol

IL21

UniProt

Q9HBE4.3

Additional IL-21 Products

Product Documents for Recombinant Human IL-21 GMP Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Manufacturing Specifications

GMP Proteins
R&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.

R&D Systems' quality focus includes:

  • Designed, manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
  • Documented and controlled manufacturing process
  • Control of documentation and process changes by QA
  • Personnel training programs
  • Raw material inspection and vendor qualification/monitoring program
  • Validated equipment, processes and test methods
  • Equipment calibration and maintenance schedules using a Regulatory Asset Manager
  • Facility/Utilities maintenance, contamination controls, safety and pest control programs
  • Material review process for variances
  • Robust product stability program following relevant ICH guidelines

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their application. Each product is provided with a lot-specific Certificate of Analysis that contains the product's specifications and test results. Quality control testing may include, but is not limited to:

  • N-terminal amino acid analysis
  • SDS-PAGE purity analysis
  • Molecular weight analysis via mass spectrometry
  • Endotoxin assessment per USP <85> and Ph. Eur. 2.6.14 guidelines
  • Bioassay analysis
  • Microbial testing per USP <71> and Ph. Eur. 2.6.1 guidelines
  • Host cell protein assessment
  • Host cell DNA assessment
  • Mycoplasma assessment

Additional testing and documentation requested by the customer can be arranged at an additional cost. 

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis and St. Paul, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.


Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

  • All molecular biology procedures use animal-free media and dedicated labware.
  • Dedicated fermentors are utilized in committed animal-free areas.

Purification

  • Protein purification columns are animal-free.
  • Bulk proteins are filtered using animal-free filters.
  • Purified proteins are stored in animal-free containers.

    Please read our complete Animal-Free Statement

    Product Specific Notices for Recombinant Human IL-21 GMP Protein, CF

    Full terms and conditions of sale can be found online in the Protein Sciences Segment T&Cs at: Terms & Conditions.

    For preclinical, or clinical ex vivo use

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