Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11655-GR

R&D Systems, part of Bio-Techne
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11655-GR-050
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human IGF-I R/IGF1R Fc Chimera Protein (11655-GR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Structure / Form

Disulfide linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IGF-I R/IGF1R Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IGF-I R/IGF1R protein
Human IGF1R
(Glu31-His935)
Accession # P08069.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu31 (apha subunit), Asp741 (beta subunit)

Predicted Molecular Mass

130 kDa (single chain), 80 kDa (alpha subunit) and 49 kDa (beta subunit)

SDS-PAGE

160-180 kDa, 110-125 kDa and 70-80 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human IGF-I R/IGF1R Fc Chimera (Catalog # 11655-GR binds to Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) with an ED50 of 3.00-45.0 ng/mL.

Scientific Data Images for Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF

Recombinant Human IGF-I R/IGF1R Fc Chimera Protein Binding Activity.

In a functional ELISA, Recombinant Human IGF-I R/IGF1R Fc Chimera Protein (Catalog # 11655-GR) binds to Recombinant Human IGF-I/IGF-1 (291-G1) with an ED50 of 3.00-45.0 ng/mL.

Recombinant Human IGF-I R/IGF1R Fc Chimera Protein SDS-PAGE.

2 μg/lane of (Catalog # 11655-GR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 160-180 kDa, 110-125 kDa and 70-80 kDa, under reducing conditions.

Formulation, Preparation and Storage

11655-GR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I R/IGF1R

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

References

  1. Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.

Long Name

Insulin-like Growth Factor I Receptor

Alternate Names

CD221, IGF-1R, IGF-IR, IGF1R, IGFIR

Entrez Gene IDs

3480 (Human)

Gene Symbol

IGF1R

Additional IGF-I R/IGF1R Products

Product Documents for Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF

For research use only

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