Recombinant Human IFN-omega Protein
R&D Systems, part of Bio-Techne | Catalog # 11395-1
Key Product Details
- R&D Systems E. coli-derived Recombinant Human IFN-omega Protein (11395-1)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Source
E. coli-derived human IFN-omega protein
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass
20 kDa
Activity
Interferon was titrated with the use of the cytopathic effect inhibition assay as
described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons,"
J. Virol. 37, 755-758;
Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for
Interferon," in
Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral
assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. As there
is no international standard for human interferon omega, the units are determined with respect to the international
reference standard for human interferon alpha A (Hu-IFN-
alphaA) provided by the National Institutes of Health [see Pestka, S.
(1986) "Interferon Standards and General Abbreviations," in
Methods in Enzymology (S. Pestka, ed.), Academic Press,
New York 119, 14-23]. Unit of activity measured on human lung carcinoma cell line A549 with encephalomyocarditis virus
(EMCV).
Formulation, Preparation and Storage
11395-1
| Formulation | Phosphate buffered saline (PBS) containing 2% bovine serum albumin (BSA) |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Background: IFN-omega
Interferon-omega (IFN-omega), a type I interferon, is a monomeric glycoprotein distantly related in structure to IFN-alpha and IFN-beta, but unrelated to IFN-gamma. The human type I interferon receptor complex, which mediates the biological activity of IFN-alpha and IFN-beta, also binds IFN-omega. The complex is composed of a 100 kDa ligand-binding subunit (IFN-alpha R1) and a 125 kDa subunit (IFN-alpha R2) that is involved both in ligand binding and signal transduction. IFN-omega is secreted by virus-infected leukocytes.
Long Name
Interferon omega
Alternate Names
IFNomega, IFNW1
Entrez Gene IDs
3467 (Human)
Gene Symbol
IFNW1
UniProt
Additional IFN-omega Products
Product Documents for Recombinant Human IFN-omega Protein
Product Specific Notices for Recombinant Human IFN-omega Protein
For research use only
Loading...
Loading...