Recombinant Human IFN-alpha 4b (alpha 4) Protein
R&D Systems, part of Bio-Techne | Catalog # 11180-1
Key Product Details
- R&D Systems E. coli-derived Recombinant Human IFN-alpha 4b (alpha 4) Protein (11180-1)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Source
E. coli-derived human IFN-alpha 4 protein
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass
19.4 kDa
Activity
Interferon was titrated with the use of the cytopathic effect inhibition assay as listed:
Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay.
Human (A549/EMCV) – performed as described [Budd, et al. (1985) Canc. Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ~1 U/ml.
Formulation, Preparation and Storage
11180-1
| Formulation | Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA) |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Background: IFN-alpha 4
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Long Name
Interferon alpha 4
Alternate Names
IFNA4, IFNalpha 4
Gene Symbol
IFNA4
UniProt
Additional IFN-alpha 4 Products
Product Documents for Recombinant Human IFN-alpha 4b (alpha 4) Protein
Product Specific Notices for Recombinant Human IFN-alpha 4b (alpha 4) Protein
For research use only
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