Recombinant Human His6-UBE2N/UBE2V2 Complex Protein, CF
R&D Systems, part of Bio-Techne | Catalog # E2-666
Discontinued Product
E2-666 has been discontinued.
View all UBE2N/UBE2V2 Complex products.
Key Product Details
Source
E. coli
Accession #
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
E. coli-derived human UBE2N/UBE2V2 Complex protein
Contains an N-terminal 6-His tag
Accession # P61088 (UBE2N)/Q15819 (UBE2V2)
Contains an N-terminal 6-His tag
Accession # P61088 (UBE2N)/Q15819 (UBE2V2)
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
18 kDa (UBE2N), 17 kDa (UBE2V2)
Activity
Recombinant Human His6-UBE2N/UBE2V2 Complex is a member of the Ubiquitin conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-UBE2N/UBE2V2 Complex concentration of 0.1-1 μM.
Formulation, Preparation and Storage
E2-666
| Formulation | 0.88 mg/ml (25 μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 2 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: UBE2N/UBE2V2 Complex
Ubiquitin conjugating Enzyme E2N (UBE2N), also known as Ubiquitin conjugating Enzyme 13 (Ubc13), forms a functional complex with the catalytically inactive UBE2V2 (human homologue of yeast MMS2) protein (1). Human UBE2N/Ubc13 shares 100% and 99% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively, while human UBE2V2 shares 99% aa sequence identity with its mouse and rat orthologs. The UBE2N/UBE2V2 Complex functions with Ubiquitin ligases (E3s), including RNF111 and RNF8, to synthesize Lys63-linked Ubiquitin chains (2,3) that can either be unanchored or attached to target proteins (4, 5). The UBE2N/UBE2V2 complex has important roles in facilitating responses to various forms of DNA damage (2, 6).
References
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Hofmann R.M.& Pickart C.M. (1999) Cell 96: 645
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Poulsen, S.L et al. (2013) Journ Cell Biol. 201: 797
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Campbell, S.J. et al. (2012) Journ. Biol. Chem. 287: 23900
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Xia, Z.P. et al. (2009) Nature 461:114.
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Liu, C. et al. (2009) Sci. Signal. 2:ra63.
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Wen, R. et al. (2012) DNA Repair 11: 157
UniProt
Product Documents for Recombinant Human His6-UBE2N/UBE2V2 Complex Protein, CF
Product Specific Notices for Recombinant Human His6-UBE2N/UBE2V2 Complex Protein, CF
For research use only
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