Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11078

R&D Systems, part of Bio-Techne
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AVI11078-050
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein (AVI11078)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Structure / Form

Disulfide linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all Glypican 3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Glypican 3 protein
Human Glypican-3
(Gln25-His559)
Accession # P51654.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln25

Predicted Molecular Mass

89 kDa

SDS-PAGE

>190 kDa, under non-reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human Glypican 3 Fc Chimera Avi-tag (Catalog # AVI11078) binds to Recombinant Human FGF basic/FGF2/bFGF (Catalog # 233-FB/CF) with an ED50 of 60.0-720 ng/mL.

Scientific Data Images for Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein Binding Activity.

In a functional ELISA, Biotinylated Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein (Catalog # AVI11078) binds to Recombinant Human FGF basic/FGF2/bFGF (233-FB/CF) with an ED50 of 60.0-720 ng/mL.

Biotinylated Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein (Catalog # AVI11078) was resolved with SDS-PAGE under non-reducing (NR) condition and visualized by Coomassie® Blue staining, showing bands at >190 kDa.

Formulation, Preparation and Storage

AVI11078
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 3

Glypican 3 (GPC3), also known as OCI5 and MXR7, is a member of the heparan sulfate proteoglycan (HSPG) family (1). In mammals, six glypican family members have been identified, all sharing a structurally common extracellular domain (ECD) with a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C‑terminal GPI attachment site. The ECD of GPC3 can be cleaved by furin to produce two subunits that are linked by disulfide bonds: a 40 kDa N‑terminal alpha subunit that can be secreted into the blood and a 30 kDa membrane-bound C‑terminal beta subunit containing two HS glycan chains (1-3). Within ECD, human GPC3 shares 96% amino acid sequence identity with both mouse and rat GPC3. Several isoforms of GPC3 due to alternative splicing have been reported (1). GPC3 is widely expressed on the membrane of various embryonic cells, but not on those in adult liver, and is involved in the regulation of growth and development of the body (4). GPC3 is over-expressed in hepatocellular carcinomas and binding of GPC3 to CD81 promotes development of carcinomas by activation of Hippo pathways in hepatocytes (5). GPC3 is an important biomarker present in the serum of hepatocellular carcinoma patients, which distinguishes benign from cancerous nodules (6). Loss-of-function mutations in GPC3 are associated with Simpson-Golabi-Behmel (SGB) syndrome, a condition characterized by tissue overgrowth (dysmorphogenesis) (7). Our Avi-tag Biotinylated Recombinant  Glypican 3  features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Ho, M. and Kim, H. (2012) Eur. J. Cancer. 47:333.
  2. Haruyama, Y. and Kataoka, H. (2016) World J. Gastroenterol. 22:275.
  3. Shimizu, Y. et al. (2019) Front Oncol. 9:248.
  4. Gonzales, A.D. et al. (1998) J. Cell Biol. 141:1407.
  5. Xue, Y. et al. (2018) Am J Pathol. 188(6):1469.
  6. Ge, S. et al. (2018) Filmus, Int J Clin Exp Pathol. 11(12):5774.
  7. Pilia, G. et al. (1996) Nature Genet.12:241.

Alternate Names

GPC3

Entrez Gene IDs

2719 (Human); 14734 (Mouse); 25236 (Rat); 102137748 (Cynomolgus Monkey)

Gene Symbol

GPC3

Additional Glypican 3 Products

Product Documents for Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Glypican 3 Fc Chimera Avi-tag Protein, CF

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