Recombinant Human GDNF Protein, CF
R&D Systems, part of Bio-Techne | Catalog # BT-GDNF
Key Product Details
Source
E. coli
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
E. coli-derived human GDNF protein
Gly113-Ile211
Gly113-Ile211
Purity
>97%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly113 & Met
Predicted Molecular Mass
16 kDa
SDS-PAGE
8 kDa, under reducing conditions.
Activity
Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells.
The ED50 for this effect is 0.750-9.00 ng/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 Fc Chimera (Catalog # 714‑GR).
The ED50 for this effect is 0.750-9.00 ng/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 Fc Chimera (Catalog # 714‑GR).
Scientific Data Images for Recombinant Human GDNF Protein, CF
Equivalent Bioactivity of GMP, Animal-Free, and RUO grades of Recombinant Human GDNF.
Equivalent bioactivity of GMP (BT-GDNF-GMP), Animal-Free (BT-GDNF-AFL) and RUO (Catalog # BT-GDNF) grades of Recombinant Human GDNF as measured in a cell proliferation assay using the cell proliferation of SH-SY5Y cells (orange, green, red, respectively).
Recombinant Human GDNF Protein Bioactivity.
Recombinant Human GDNF Protein (Catalog # BT-GDNF) stimulates proliferation in the SH-SY5Y human neuroblastoma cell line. The ED50 for this effect is 0.750-9.00 ng/mL in the presence of Recombinant Human GFRa-1/GDNF Ra-1 Fc Chimera Protein (714-GR).
Recombinant Human GDNFProtein SDS-PAGE.
2 μg/lane ofRecombinant Human GDNFProtein (Catalog # BT-GDNF)was resolved with SDS-PAGEunder reducing (R) and nonreducing (NR) conditions andvisualized by Coomassie® Bluestaining, showing a band at 8 kDaunder reducing conditions.Formulation, Preparation and Storage
BT-GDNF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute the 10 μg size at 100 μg/mL in water. Reconstitute all other sizes at 500 μg/mL in water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: GDNF
Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.
Long Name
Glial Cell line-derived Growth Factor
Alternate Names
ATF, HFB1-GDNF, HGDNF, HSCR3
Gene Symbol
GDNF
UniProt
Additional GDNF Products
Product Documents for Recombinant Human GDNF Protein, CF
Product Specific Notices for Recombinant Human GDNF Protein, CF
For research use only
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