Recombinant Human Angiopoietin-like 6 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8466-AN/CF

R&D Systems, part of Bio-Techne
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8466-AN-025/CF
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Key Product Details

Source

CHO

Accession #

Q8NI99

Structure / Form

Disulfide-linked homo-oligomer

Conjugate

Unconjugated

Applications

Bioactivity

View all Angiopoietin-like Protein 6/ANGPTL6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 6/ANGPTL6 protein
Ala20-Leu470, with an N-terminal c-Myc tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu (c-Myc tag)

Predicted Molecular Mass

51 kDa

SDS-PAGE

58-69 kDa and 31-41 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Angiopoietin‑like Protein 6/ANGPTL6 (Catalog # 8466-AN/CF) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4) binds with an ED50 of 30.0-360 ng/mL.

Scientific Data Images for Recombinant Human Angiopoietin-like 6 Protein, CF

ANGPTL6 Promotes Rat Liver Mononuclear Cell Expansion.

Recombinant Human Angiopoietin‑like Protein 6/ANGPTL6 Binding Activity.

When Recombinant Human Angiopoietin‑like Protein 6/ANGPTL6 (Catalog # 8466-AN/CF) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (2078-T4) binds with an ED50 of 30.0-360 ng/mL.

Formulation, Preparation and Storage

8466-AN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CHAPS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 6/ANGPTL6

Angiopoietin-like Protein 6 (ANGPTL6), also known as angiopoietin-related growth factor (AGF), is a secreted 50 kDa protein that contains a coiled-coil domain and a fibrinogen-like domain (1, 2). Mature human ANGPTL6 shares 75% sequence identity with both mouse and rat ANGPTL6. ANGPTL6 is highly expressed in hepatocytes and is detectable in serum (1, 2). Within the liver, secreted ANGPTL6 inhibits gluconeogenesis and promotes insulin sensitivity and energy expenditure (3, 4). Circulating ANGPTL6 promotes the chemotaxis of vascular endothelial cells resulting in increased vascular permeability and angiogenesis (5, 6). An integrin­-binding RGD motif located within the fibrinogen-like domain of ANGPTL6, enables it to promote skin wound healing by mediating the adhesion and migration of keratinocytes, fibroblasts, and endothelial cells (5, 7). Elevated levels of ANGPTL6 in serum are associated with metabolic syndrome, diabetes, and pre-eclampsia (8-11). Serum ANGPTL6 levels are also elevated during obesity (4, 12). ANGPTL6 is reported to promote the survival and expansion of hematopoietic stem cells (13). ANGPTL6 is produced by several hematopoietic cell types including megakaryocytes, platelets, mast cells, and uterine NK cells (7).

References

  1. Oike, Y. and M. Tabata (2009) Circ. J. 73:2192.
  2. Kadomatsu, T. et al. (2011) FEBS J. 278:559.
  3. Kitazawa, M. et al. (2007) J. Pharmacol. Exp. Ther. 323:787.
  4. Oike, Y. et al. (2005) Nat. Med. 11:400.
  5. Oike, Y. et al. (2003) Proc. Natl. Acad. Sci. U S A 100:9494.
  6. Okazaki, H. et al. (2012) J. Dermatol. 39:366.
  7. Zhang, Y. et al. (2006) Biochem. Biophys. Res. Commun. 347:100.
  8. Legry, V. et al. (2009) Diabetes. Metab. 35:287.
  9. Namkung, J. et al. (2011) Metabolism 60:564.
  10. Stepan, H. et al. (2009) Am. J. Hypertens. 22:314.
  11. Ebert, T. et al. (2009) Metabolism 58:547.
  12. Ebert, T. et al. (2014) Horm. Metab. Res. 46:685.
  13. Akhter, S. et al. (2013) Protein Cell 4:220.

Alternate Names

AGF, Angiopoietin like Protein 6, ANGPTL6, ARP5

Entrez Gene IDs

83854 (Human); 70726 (Mouse); 298698 (Rat)

Gene Symbol

ANGPTL6

UniProt

Q8NI99

Additional Angiopoietin-like Protein 6/ANGPTL6 Products

Product Documents for Recombinant Human Angiopoietin-like 6 Protein, CF

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Product Specific Notices for Recombinant Human Angiopoietin-like 6 Protein, CF

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