Recombinant Human Activated Coagulation Factor Xa, ACFP

R&D Systems, part of Bio-Techne | Catalog # ACFP1063

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ACFP1063
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Key Product Details

Source

Sf 9 (baculovirus)

Accession #

P00742

Structure / Form

Active Form. Recombinant Human Coagulation Factor Xa Animal Component Free is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks the His tag.

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Coagulation Factor X Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 9 (baculovirus)-derived human Coagulation Factor X protein
Met1-Lys488, with a C-terminal 10-His tag
Proform Factor X was expressed, purified, activated and further purified to yield Factor Xa. Produced in an animal component free process (ACFP).

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe80, Tyr84, & Ile235

Predicted Molecular Mass

16.6 kDa & 30 kDa

SDS-PAGE

12-15 kDa and 30-36 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >800 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

ACFP1063
Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl and CaCl2.
Reconstitution Reconstitute at 100 μg/mL in sterile 25 mM MES, 150 mM NaCl and 5 mM CaCl2, pH 6.0
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor X

As the only known physiological activator of thrombin, Factor X is a vitamin K-dependent plasma protease that plays a pivotal role in blood coagulation. Human Factor X is initially synthesized in the liver as a single-chain precursor of 488 amino acid residues with a signal peptide and a pro region (residues 1‑40). Both the intrinsic and extrinsic pathways activate Factor X to Xa, which consists of light (residues 41‑179) and heavy (residues 235‑488) chains linked by a disulfide bond. The light chain contains a Gla and two EGF‑like domains and the heavy chain corresponds to the serine protease domain. The full-length human Factor X was expressed and the pro enzyme was purified and activated. The active protease (rhFactor Xa) was further purified and analyzed for its activity towards either peptides or proteins containing a Xa cleavage site. In addition to the activity described in the Activity Assay Protocol, rhFactor Xa also can be used to cleave fusion proteins containing a Factor Xa cleavage site. The conditions for the optimal cleavage of a particular fusion protein, such as the molar ratio between rhFXa and the protein and time and temperature of incubation, are protein-dependent and need to be individually determined.

Alternate Names

Cf10, F10, FX, FXa, Prothrombinase, Stuart Factor

Entrez Gene IDs

2159 (Human); 14058 (Mouse); 29243 (Rat)

Gene Symbol

F10

UniProt

P00742

Additional Coagulation Factor X Products

Product Documents for Recombinant Human Activated Coagulation Factor Xa, ACFP

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human Activated Coagulation Factor Xa, ACFP

For research use or further manufacturing only

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