Recombinant Human Rab27a His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44380

Novus Biologicals, part of Bio-Techne
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Rab27a Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-221 of Human Rab27a

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSDGDYDYLI KFLALGDSGV GKTSVLYQYT DGKFNSKFIT TVGIDFREKR VVYRASGPDG ATGRGQRIHL QLWDTAGQER FRSLTTAFFR DAMGFLLLFD LTNEQSFLNV RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RVVKEEEAIA LAEKYGIPYF ETSAANGTNI SQAIEMLLDL IMKRMERCVD KSWIPEGVVR SNGHASTDQL SEEKEKGACG C

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Rab27a His Protein

SDS-PAGE: Recombinant Human Rab27a His Protein [NBP1-44380]

SDS-PAGE: Recombinant Human Rab27a His Protein [NBP1-44380]

SDS-Page: Recombinant Human Rab27a Protein [NBP1-44380] - RAB27A, 27 kDa (241aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-44380
Formulation 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 20% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rab27a

Ras-related protein Rab-27A, also known as RAB27A, belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Also, RAB27A regulates the cytotoxic granule exocytosis and affect T-lymphocyte and macrophage-activation. It is widely expressed with significant expression in pancreatic islets and pituitary tissue, and low expression in brain. Defects in RAB27A cause Griscelli syndrome type 2 (GS2). Recombinant human RAB27A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

RAs Genes from Brain Protein 27A

Alternate Names

GS2, HsT18676, RAM

Gene Symbol

RAB27A

Additional Rab27a Products

Product Documents for Recombinant Human Rab27a His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Rab27a His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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