Recombinant Human RAB10 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23392

Novus Biologicals, part of Bio-Techne
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NBP2-23392
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Western Blot, SDS-PAGE

View all RAB10 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-200 of Human RAB10

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAKKTYDLLF KLLLIGDSGV GKTCVLFRFS DDAFNTTFIS TIGIDFKIKT VELQGKKIKL QIWDTAGQER FHTITTSYYR GAMGIMLVYD ITNGKSFENI SKWLRNIDEH ANEDVERMLL GNKCDMDDKR VVPKGKGEQI AREHGIRFFE TSAKANINIE KAFLTLAEDI LRKTPVKEPN SENVDISSGG GVTGWKSKCC

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

24.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays. Use in WB reported in scientific literature (PMID:10.1242/dmm.048017)

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human RAB10 His Protein

SDS-PAGE: Recombinant Human RAB10 His Protein [NBP2-23392]

SDS-PAGE: Recombinant Human RAB10 His Protein [NBP2-23392]

SDS-Page: Recombinant Human RAB10 Protein [NBP2-23392]

Formulation, Preparation and Storage

NBP2-23392
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAB10

RAB10 is a member of the Ras superfamily of small GTP-binding proteins. This protein is a key regulator of endocytic recycling, especially in the basolateral recycling pathways of polarized epithelial cells. It is required for the recruitment of CNT-1 to endosomal membranes in the intestinal epithelium. Recombinant human RAB10 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

GTP-binding protein RAB10, RAB10, member RAS oncogene family, ras-related GTP-binding protein, ras-related protein Rab-10

Gene Symbol

RAB10

Additional RAB10 Products

Product Documents for Recombinant Human RAB10 His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human RAB10 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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