Recombinant Human PDGF-AA Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07102
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Description
A disulfide-linked homodimeric protein containing two 126 amino acid polypeptide chains corresponding to PDGF-AA. However it migrates with an apparent molecular mass of 336 kDa in SDS-PAGE Source: E. coli
Amino Acid Sequence: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT
Purity
>95%, by SDS-PAGE
Endotoxin Level
Less than 0.1 EU/ug of PDGF-AA as determined by LAL method.
Predicted Molecular Mass
28.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Biological activity measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for tis effect is 50-200 ng/mL in a fluorometric assay using a redox sensitive dye.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP3-07102
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 300 mM NaCl, pH 6.0. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Briefly centrifuge vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to 0.1-1.0 mg/mL. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: PDGF-AA
PDGF-AA (Platelet-Derived Growth Factor AA) is a widely expressed protein that forms disulfide linked homodimers or heterodimers with PDGF-B. PDGF-AA is expressed by monocytes, macrophages, fibroblasts, vascular smooth muscle cells, and vascular endothelial cells. It acts principally as a chemoattractant and inducer of protein synthesis in multiple cell types. PDGF-AA binds and signals through homodimers of the receptor tyrosine kinase PDGF R alpha which itself associates with FGF R1 and Neuropilin-1. PDGF-AA additionally signals through heterodimers of PDGF R alpha and PDGF R beta.
Long Name
Platelet-derived Growth Factor AA
Alternate Names
PDGFAA
Gene Symbol
PDGFA
Additional PDGF-AA Products
Product Documents for Recombinant Human PDGF-AA Protein
Product Specific Notices for Recombinant Human PDGF-AA Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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