Recombinant E. coli nfnB His Protein

Name: Recombinant E. coli nfnB His Protein
Brand: Novus Biologicals
SKU: NBP1-46035

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46035

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all nfnB Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-217 of E.coli nfnB

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDIISVALKR HSTKAFDASK KLTPEQAEQI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVFCAKTAM DDVWLKLVVD QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHDDAEWMA KQVYLNVGNF LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYTSLVVVP VGHHSVEDFN ATLPKSRLPQ NITLTEV

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli nfnB His Protein

Western Blot: Recombinant E. coli nfnB His Protein [NBP1-46035]

Western Blot: nfnB Protein [NBP1-46035] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-46035

Formulation	20 mM Tris-HCl buffer (pH8.0), 50 mM Nacl, 1 mM DTT, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: nfnB

NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E.coli NFNB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

dprA, nfsB, nfsI, ntr

Additional nfnB Products

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COA

Product Specific Notices for Recombinant E. coli nfnB His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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