Recombinant E. coli MDH1 His Protein

Name: Recombinant E. coli MDH1 His Protein
Brand: Novus Biologicals
SKU: NBP1-99038

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-99038

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all MDH1 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-312 of E.coli MDH1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

34.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 1000 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli MDH1 His Protein

SDS-PAGE: Recombinant E. coli MDH1 His Protein [NBP1-99038]

SDS-Page: Recombinant E. coli MDH1 Protein [NBP1-99038] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-99038

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 50 mM NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MDH1

Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Recombinant E.coli mdh protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

malate dehydrogenase 1

Alternate Names

DEE88, EIEE88, HEL-S-32, KAR, MDH-s, MDHA, MGC:1375, MOR2

Gene Symbol

MDH1

Additional MDH1 Products

Product Documents for Recombinant E. coli MDH1 His Protein

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download SDS-PAGE Protocol

COA

Product Specific Notices for Recombinant E. coli MDH1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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