Irisin/FNDC5 Recombinant Protein Antigen
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14024PEP
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Antibody Competition
Product Specifications
Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human FNDC5.
Source: E. coli
Amino Acid Sequence: EDTEYIVHVQAISIQGQSPASEPVLFKTPREAEKMASKNKDEVTMKEMGRNQQLR
Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)
This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.
Purity
>80% by SDS-PAGE and Coomassie blue staining
Predicted Molecular Mass
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications
Antibody Competition (10 - 100 molar excess)
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-14024.
It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.
For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com
Protein / Peptide Type
Recombinant Protein Antigen
Formulation, Preparation and Storage
NBP2-14024PEP
| Formulation | PBS and 1M Urea, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20C. Avoid freeze-thaw cycles. |
Background: Irisin/FNDC5
Irisin, also known as FNDC5, is a peptide hormone that is upregulated in skeletal muscle and subcutaneous adipose tissue during exercise. It induces expression of the mitochondrial proteins PPAR-gamma coactivator 1 alpha (PGC1a) and uncoupling protein-1 (UCP1). It contributes to the regulation of diabetes and obesity by inducing the transition of white adipose tissue into more metabolically active beige adipose tissue. Irisin also regulates neuronal cell differentiation, neurite outgrowth, and osteoblast differentiation.
Long Name
Fibronectin Type III Domain Containing 5
Alternate Names
FNDC5, FRCP2
Gene Symbol
FNDC5
Additional Irisin/FNDC5 Products
Product Documents for Irisin/FNDC5 Recombinant Protein Antigen
Product Specific Notices for Irisin/FNDC5 Recombinant Protein Antigen
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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