Recombinant Human FIH-1/HIF-1AN Protein

Name: Recombinant Human FIH-1/HIF-1AN Protein
Brand: Novus Biologicals
SKU: NBC1-22596

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-22596

Submit a review

Product Datasheet / COA

100% Guarantee

Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

SDS-PAGE

View all FIH-1/HIF-1AN Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-349 of Human FIH-1/HIF-1AN

Source: E.coli

Amino Acid Sequence: MAATAAEAVA SGSGEPREEA GALGPAWDES QLRSYSFPTR PIPRLSQSDP RAEELIENEE PVVLTDTNLV YPALKWDLEY LQENIGNGDF SVYSASTHKF LYYDEKKMAN FQNFKPRSNR EEMKFHEFVE KLQDIQQRGG EERLYLQQTL NDTVGRKIVM DFLGFNWNWI NKQQGKRGWG QLTSNLLLIG MEGNVTPAHY DEQQNFFAQI KGYKRCILFP PDQFECLYPY PVHHPCDRQS QVDFDNPDYE RFPNFQNVVG YETVVGPGDV LYIPMYWWHH IESLLNGGIT ITVNFWYKGA PTPKRIEYPL KAHQKVAIMR NIEKMLGEAL GNPQEVGPLL NTMIKGRYN

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

40.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human FIH-1/HIF-1AN Protein

SDS-PAGE: Recombinant Human FIH-1/HIF-1AN Protein [NBC1-22596]

SDS-Page: Recombinant Human FIH-1/HIF-1AN Protein [NBC1-22596] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight: 40.2 kDa (349 aa), confirmed by MALDI-TOF. Concentration: 1 mg/ml (determined by Bradford assay)

Formulation, Preparation and Storage

NBC1-22596

Formulation	20 mM Tris-HCl buffer (pH 8.0)
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FIH-1/HIF-1AN

Description: Hypoxia-inducible factor 1, alpha subunit inhibitor (HIF1AN), a member of the Fe2+ and 2-oxoglutarate-dependent dioxygenase superfamily, is protein that hydoxylates a specific asparagine residue (Asn-803) within the HIF1alpha C-terminal transactivation domain. In normoxia, the HIF1AN-mediated hydroxylation of the HIF1alpha transactivation domain results in blockage of the HIF1alpha-p300/CBP interaction and represses transcriptional activity of HIF1alpha-targeted genes. Recombinant human HIF1AN protein was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Factor Inhibiting HIF-1

Alternate Names

FIH1, HIF-1AN, HIF1AN, Peptide-aspartate beta-dioxygenase

Gene Symbol

HIF1AN

Additional FIH-1/HIF-1AN Products

Product Documents for Recombinant Human FIH-1/HIF-1AN Protein

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Recombinant Human FIH-1/HIF-1AN Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations
Reviews