Recombinant Human FGF acidic/FGF1 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61310
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Western Blot, Bioactivity
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 154 amino acids corresponding to FGF acidic/FGF1 Source: E. coli
Uniprot ID: P05230
Amino Acid Sequence: AEGEITTFTA LTEKFNLPPG NYKKPKLLYC SNGGHFLRIL PDGTVDGTRD RSDQHIQLQL SAESVGEVYI KSTETGQYLA MDTDGLLYGS QTPNEECLFL ERLEENHYNT YISKKHAEKN WFVGLKKNGS CKRGPRTHYG QKAILFLPLP VSSD
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of FGF acidic/FGF1 as determined by LAL method.
Predicted Molecular Mass
17.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
FGF acidic/FGF1 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg in the presence of 10 ug/ml of heparin.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human FGF acidic/FGF1 Protein
![SDS-PAGE: Recombinant Human FGF acidic/FGF1 Protein [NBP2-61310]](https://resources.bio-techne.com/images/products/Recombinant-Human-FGF-acidic-FGF1-Protein-SDS-Page-NBP2-61310-img0002.jpg "SDS-PAGE: Recombinant Human FGF acidic/FGF1 Protein [NBP2-61310]")
SDS-PAGE: Recombinant Human FGF acidic/FGF1 Protein [NBP2-61310]
SDS-Page: Recombinant Human FGF acidic/FGF1 Protein [NBP2-61310]Formulation, Preparation and Storage
NBP2-61310
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 2 mM EDTA, 0.5 mM DTT. |
| Preservative | 5% Trehalose |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: FGF acidic/FGF1
FGF acidic/FGF1, also known as ECGF and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF1 is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF1 with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF1 can translocate to the cytosol and to the nucleus. Intracellular FGF1 functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.
Long Name
Fibroblast Growth Factor acidic
Alternate Names
alpha-ECGF, beta-ECGF, FGF acidic, FGF-1, HBGF-1
Gene Symbol
FGF1
Additional FGF acidic/FGF1 Products
Product Documents for Recombinant Human FGF acidic/FGF1 Protein
Product Specific Notices for Recombinant Human FGF acidic/FGF1 Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...