Recombinant Human DC8 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23295

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NBP2-23295
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all DC8 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-281 of Human DC8

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGSPEL VVLDPPWDKE LAAGTESQAL VSATPREDFR VRCTSKRAVT EMLQLCGRFV QKLGDALPEE IREPALRDAQ WTFESAVQEN ISINGQAWQE ASDNCFMDSD IKVLEDQFDE IIVDIATKRK QYPRKILECV IKTIKAKQEI LKQYHPVVHP LDLKYDPDPA PHMENLKCRG ETVAKEISEA MKSLPALIEQ GEGFSQVLRM QPVIHLQRIH QEVFSSCHRK PDAKPENFIT QIETTPTETA SRKTSDMVLK RKQTKDCPQR KWYPLRPKKI NLDT

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

34.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human DC8 His Protein

SDS-PAGE: Recombinant Human DC8 His Protein [NBP2-23295]

SDS-PAGE: Recombinant Human DC8 His Protein [NBP2-23295]

SDS-Page: DC8 Protein [NBP2-23295]

Formulation, Preparation and Storage

NBP2-23295
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DC8

NSL1 is a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human NSL1 protein, fused to His-tag at N-terminus, was expressed in E.coli .

Alternate Names

C1orf48, chromosome 1 open reading frame 48, DC8, DKFZP566O1646, kinetochore-associated protein NSL1 homolog, MIS14, NSL1, MIND kinetochore complex component, homolog (S. cerevisiae)

Gene Symbol

NSL1

Additional DC8 Products

Product Documents for Recombinant Human DC8 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human DC8 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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