Recombinant Feline Cystatin B/Stefin B Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-57604

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NBP3-57604-5ug
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Key Product Details

Conjugate

Unconjugated

Applications

Western Blot, ELISA, Cell Culture

View all Cystatin B Proteins and Enzymes »

Product Specifications

Description

A recombinant protein corresponding to 98 aa of Feline Cystatin B/Stefin B.

Source: Yeast

Amino Acid Sequence: MMCGAPSATQ PATAETQAIA DQVKPQLEEQ ENKKYTTFKA VEFRSQVVAG RNYFIKVQVD DDEFVHIRVF QSLPHENKPL ALSSYQTHKA RHDELAYF (98)

Stable for up to 12 months from date of receipt at -20C. Stable for 3 months when stored in working aliquots with a carrier protein at -20C. Avoid repeated freeze/thaw cycles.

Purity

>98%, by SDS-PAGE

Endotoxin Level

No affinity tags & naturally endotoxin-free

Predicted Molecular Mass

11.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Enzyme-linked Immunosorbent Spot (ELISPOT)
ELISA Standard (Matched Pair) (ELISA(Sta))

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP3-57604
Formulation Lyophilized in 1-5X PBS containing 10% Trehalose
Preservative 10% Trehalose
Reconstitution Reconstitute in sterile PBS with at least 0.1% carrier protein
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: Cystatin B

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1).

Alternate Names

CSTB, Stefin B

Gene Symbol

CSTB

Additional Cystatin B Products

Product Documents for Recombinant Feline Cystatin B/Stefin B Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Recombinant Feline Cystatin B/Stefin B Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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