Collagen VI Native Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97270
Key Product Details
Product Specifications
Description
Native Human Collagen VI alpha 1
Source: Human placenta (Accession #:P12109)
Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use.
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Source: Human placenta (Accession #:P12109)
Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use.
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Purity
Chromatography
Applications
Immunohistochemistry (1:100-1:500)
Western Blot (1:1000)
ELISA (1:10000)
Western Blot (1:1000)
ELISA (1:10000)
Application Notes
This product has been tested in SDS-Page and is used as a control in ELISA and western blot. Collagen type VI is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.
Protein / Peptide Type
Native Protein
Scientific Data Images for Collagen VI Native Protein
![Collagen VI alpha 1 Native Protein SDS-Page: Collagen VI alpha 1 Native Protein [NBP1-97270] -](https://resources.bio-techne.com/images/products/nbp1-97270_collagen-vi-alpha-1-native-protein-231202523584712.jpg "SDS-Page: Collagen VI alpha 1 Native Protein [NBP1-97270] -")
SDS-Page: Collagen VI alpha 1 Native Protein [NBP1-97270] -
SDS-Page: Collagen VI alpha 1 Native Protein [NBP1-97270] - SDS-PAGE results of Collagen VI alpha 1 Native Protein. Lane: 1) Opal prestain molecular weight marker, 2) blank, 3) Collagen VI alpha 1 Native Protein [10µg]. Coomassie stained.![Collagen VI alpha 1 Native Protein Western Blot: Collagen VI alpha 1 Native Protein [NBP1-97270] -](https://resources.bio-techne.com/images/products/nbp1-97270_collagen-vi-alpha-1-native-protein-231202523584720.jpg "Western Blot: Collagen VI alpha 1 Native Protein [NBP1-97270] -")
Western Blot: Collagen VI alpha 1 Native Protein [NBP1-97270] -
Western Blot: Collagen VI alpha 1 Native Protein [NBP1-97270] - Western blot results using Collagen VI alpha 1 Native Protein. Lane: 1) Human Collagen Type VI reduced and boiled, 3) Human Collagen Type VI reduced and not boiled,5) Opal pre-stained molecular weight marker, 7) Human Collagen Type VI not reduced and boiled, 9) Human Collagen Type VI not reduced and not boiled. Lanes 2, 4, 6, 8 blank. Load: 10µg. Primary Antibody: Collagen VI alpha 1 Native Protein 1:500 overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit IgG HRP conjugated 1:40,000 at RT for 30mins. Blocking: BlockOut® Universal Blocking Buffer at RT for 1hr.![Collagen VI alpha 1 Native Protein Dot Blot: Collagen VI alpha 1 Native Protein [NBP1-97270] -](https://resources.bio-techne.com/images/products/nbp1-97270_collagen-vi-alpha-1-native-protein-23120252359820.jpg "Dot Blot: Collagen VI alpha 1 Native Protein [NBP1-97270] -")
Dot Blot: Collagen VI alpha 1 Native Protein [NBP1-97270] -
Dot Blot: Collagen VI alpha 1 Native Protein [NBP1-97270] - Collagen VI alpha 1 Native Protein. Sample: A) Human Collagen VI, B) Bovine/Human Collagen VI.Load: 1) 1000ng, 2) 333.33ng, 3) 111.11ng, 4) 37.03ng, 5) 12.35ng.Primary Antibody: Rabbit Anti-Collagen VI antibody at 5µg/mL at RT for 1hr. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated 1:40,000 at RT for 30mins. Blocking: BlockOut® Universal Blocking Buffer at RT for 30mins.Formulation, Preparation and Storage
NBP1-97270
| Preparation Method | Prepared from Human Placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. |
| Formulation | 0.5 M Acetic Acid |
| Preservative | 0.01% Sodium Azide |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C. |
Background: Collagen VI
Gene Symbol
COL6A1
UniProt
Additional Collagen VI Products
Product Documents for Collagen VI Native Protein
Product Specific Notices for Collagen VI Native Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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