Recombinant Human Bmf T7 Protein

Name: Recombinant Human Bmf T7 Protein
Brand: Novus Biologicals
SKU: NBC1-28000

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-28000

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

SDS-PAGE

View all BMF Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal T7-tag and corresponding to the amino acids 1-129 of Human Bmf

Source: E.coli

Amino Acid Sequence: MASMTGGQQM GRGSHMEPSQ CVEELEDDVF QPEDGEPVTQ PGSLLSADLF AQSLLDCPLS RLQLFPLTHC CGPGLRPTSQ EDKATQTLSP ASPSQGVMLP CGVTEEPQRL FYAPAEPKSC VVADPPLPAQ PCFEWRREQE RGRP

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

15.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Bmf T7 Protein

SDS-PAGE: Recombinant Human Bmf T7 Protein [NBC1-28000]

SDS-Page: Bmf Protein [NBC1-28000] - BMF, 15.6 kDa (144aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Formulation, Preparation and Storage

NBC1-28000

Formulation	20 mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BMF

Bcl2 modifying factor, also known as BMF belongs to the Bcl2 protein family of apoptosis mediators. Bmf is constitutively expressed in many tissues. This protein contains a single Bcl2 homology domain 3 (BH3), and has been shown to bind Bcl2 proteins and function as an apoptotic activator. Also, BMF is a key molecule for histone deacetylase (HDAC) inhibitors which alters the balance between acetylation and deacetylation, significantly increasing histone acetylation, while strongly inducing apoptosis in a variety of cancer cell types. Recombinant human BMF, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Bcl-2 Modifying Factor

Alternate Names

Bcl2 modifying factor, bcl-2-modifying factor, FLJ00065

Gene Symbol

BMF

Additional BMF Products

Product Documents for Recombinant Human Bmf T7 Protein

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COA

Product Specific Notices for Recombinant Human Bmf T7 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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