Recombinant Human beta-Defensin 4/2 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34915

Novus Biologicals, part of Bio-Techne
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Key Product Details

Source

E. coli

Accession #

Q8WTQ1

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all beta-Defensin 2 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 50 amino acids corresponding to beta-Defensin 4/2 Source: E. coli

Uniprot ID: Q8WTQ1

Amino Acid Sequence: EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of beta-Defensin 4/2 as determined by LAL method.

Predicted Molecular Mass

6.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Defensin beta 4 protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human beta-Defensin 4/2 Protein

SDS-PAGE: Recombinant Human beta-Defensin 4/2 Protein [NBP2-34915]

SDS-PAGE: Recombinant Human beta-Defensin 4/2 Protein [NBP2-34915]

SDS-Page: Recombinant Human beta-Defensin 4/2 Protein [NBP2-34915]

Formulation, Preparation and Storage

NBP2-34915
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: beta-Defensin 2

Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 k Da proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. B-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human B-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because B-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca 2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane.

Long Name

Defensin 2 beta

Alternate Names

BD-2, betaDefensin 2, DEFB2, DEFB4A, DEFB4B, HBD-2, SAP-1

Gene Symbol

DEFB4A

UniProt

Q8WTQ1 (Human)

Additional beta-Defensin 2 Products

Product Documents for Recombinant Human beta-Defensin 4/2 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human beta-Defensin 4/2 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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