eEF1A2 binding protein Overexpression Lysate
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-04981
Key Product Details
Species
Human
Applications
Western Blot
Product Summary for eEF1A2 binding protein Overexpression Lysate
eEF1A2 binding protein Transient Overexpression Lysate
Expression Host: HEK293T
Plasmid: RC224460
Accession#: NM_178275
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug) and 1 vial of empty vector negative control (100ug). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml).
Product Specifications
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate.
Theoretical MW
93.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Type
Overexpression
Scientific Data Images for eEF1A2 binding protein Overexpression Lysate
![Western Blot: eEF1A2 binding protein Overexpression Lysate [NBP2-04981]](https://resources.bio-techne.com/images/products/eEF1A2-binding-protein-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04981-img0001.jpg "Western Blot: eEF1A2 binding protein Overexpression Lysate [NBP2-04981]")
Western Blot: eEF1A2 binding protein Overexpression Lysate [NBP2-04981]
Western Blot: eEF1A2 binding protein Overexpression Lysate (Adult Normal) [NBP2-04981] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eEF1A2 binding protein.Formulation, Preparation, and Storage
Formulation
RIPA buffer
Concentration
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -80C. Avoid freeze-thaw cycles.
Background: eEF1A2 binding protein
Alternate Names
DKFZp434B1231, eEF1A2 binding protein, EEF1A2-binding protein 1, EEF1A2BP1, immunoglobulin-like and fibronectin type III domain containing 1, immunoglobulin-like and fibronectin type III domain-containing protein 1, KY-interacting protein 1, KYIP1
Gene Symbol
IGFN1
Additional eEF1A2 binding protein Products
Product Documents for eEF1A2 binding protein Overexpression Lysate
Product Specific Notices for eEF1A2 binding protein Overexpression Lysate
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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