Virginia Orange, Haloalkane New
Tocris Bioscience, part of Bio-Techne | Catalog # 8959
Cell-permeable fluorogenic fluorescent dye with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag substrate
Key Product Details
Description
Cell-permeable fluorogenic fluorescent dye with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag substrate
Product Description
Key Information: Virginia Orange, Haloalkane is pH sensitive a red cell-permeable fluorogenic fluorescent dye with a chloroalkane handle.Used for: endosomal uptake assays, protein delivery experiments and evaluation of protein cargo internalization.
Application: Live-cell imaging as a self-labeling tag substrate; fluorescent microscopy and flow cytometry.
Properties and Photophysical Data: shows a high degree of fluorogenicity. Excitation and emission maxima (λ) are 555 nm and 581 nm, respectively; quantum yield = 0.40; extinction coefficient = 90,900 M-1cm-1; pH sensitive fluorescence h = 1.46. PKa = 6.25. Colourless and non-fluorescent at low pH.
Optical Data
| Emission Color | Red |
| λabs | 555 nm |
| λem | 581 nm |
| Extinction Coefficient (ε) | 90,900 M-1cm-1 |
| Quantum Yield (φ) | 0.4 |
| Cell Permeable | Yes |
| pH Sensitivity | 1.46 |
| Application | Fluorescence microscopy, flow cytometry |
Spectra Viewer
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Product Specifications
Molecular Weight
644.11
Formula
C34H36ClF2NO7
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
4-((2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)-2-(2,7-difluoro-6-hydroxy-10,10-dimethyl-3-oxo-3,10-dihydroanthracen-9-yl)benzoic acid
InChI Key
LSFLCNCAFZHNTQ-UHFFFAOYSA-N
SMILES
O=C(C1=CC=C(C(C(C2=CC(F)=C(C=C2C3(C)C)O)=C(C3=C4)C=C(F)C4=O)=C1)C(O)=O)NCCOCCOCCCCCCCl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 6.44 | 10 |
Preparing Stock Solutions
for Virginia Orange, Haloalkane
The following data is based on the product molecular weight 644.11
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 15.53 mL | 77.63 mL | 155.25 mL |
| 0.5 mM | 3.11 mL | 15.53 mL | 31.05 mL |
| 1 mM | 1.55 mL | 7.76 mL | 15.53 mL |
| 5 mM | 0.31 mL | 1.55 mL | 3.11 mL |
Calculators
Molarity Calculator
Dilution Calculator
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Background References
References are publications that support the biological activity of the product.
- Giancola Evaluation of the cytosolic uptake of HaloTag using a pH-sensitive dye. ACS Chem.Biol. 2024 PMID: 38525961
- Grimm Virginia Orange: A versatile, red-shifted fluorescein scaffold for single- and dual-input fluorogenic probes. BC Conj.Chem. 2015 PMID: 26636613
Product Specific Notices for Virginia Orange, Haloalkane
For research use only
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